Question

A 0.0000792 M sample of Compound X in a solvent has an absorbance of 0.341 at 528 nm in a 1.000-cm cuvet. The solvent alone has an absorbance of 0.065. a. What is the molar absorptivity of Compound X? b. A different sample of Compound X in the same solvent has an absorbance of 0.508. What is the concentration of this second sample?

Answer 1

Answer:

a) a = 3485 M⁻¹cm⁻¹

b) C = 0,000127 M

Explanation:

Lambert-Beer law says that there is a linear relationship between absorbance and concentration of a chemical substance. The formula is:

A = a×b×C

Where A is absorbance, a is molar absorptivity, b is path length and C is concentration.

a) In the problem Concentration is 0.0000792 M, b is 1,000cm and Absorbance is absorbance of sample-absorbance of blank: 0,341-0,065 = 0,276

Replacing:

0,276 = a×1,000cm×0,0000792M

a = 3485 M⁻¹cm⁻¹

b) As the experiment consist in the same compound in the same solvent, the molar absorptivity will be the same, a = 3485 M⁻¹cm⁻¹, path length will be 1,000cm and absorbance: 0,508-0,065 = 0,443

Replacing:

0,443 = 3485 M⁻¹cm⁻¹×1,000cm×C

C = 0,000127 M

I hope it helps!