Question

Select the procedure that will NOT help saturate a thin‑layer chromatography (TLC) developing chamber with developing solvent vapors.
(A) Cover the chamber.
(B) Gently swirl the solvent in the chamber prior to placing the TLC plate inside.
(C) Add ninhydrin (a visualization technique) to the developing solvent.
(D) Place a paper wick (like a piece of filter paper) inside the chamber.

Answer 1

Answer:

C)

Explanation:

(A) Cover the chamber. => It helps to keep the solvent in his gas state

(B) Gently swirl the solvent in the chamber prior to placing the TLC plate inside => The mechanical energy can promote the conversion from the liquid state to the gas state and help to the saturation process.

(C) Add ninhydrin (a visualization technique) to the developing solvent =>There is no change in the saturation process

(D) Place a paper wick (like a piece of filter paper) inside the chamber=>Increases the area for evaporation. The solvent can go up across the paper by capillarity a then can be evaporated increasing the saturation in the chamber.