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viva [34]
3 years ago
13

You don't know which insert you have, and the inserts are different sizes, meaning the amount needed for a 1:3 ratio is differen

t. What do you do? Why? (assume there are enough materials for more than one reaction per person).
Engineering
1 answer:
VashaNatasha [74]3 years ago
6 0

Answer:

Explanation:

For ligation process the 1:3 vector to insert ratio is the good to utilize . By considering that we can take 1 ratio of vector and 3 ratio of insert ( consider different insert size ) and take 10 different vials of ligation ( each calculated using different insert size from low to high ) and plot a graph for transformation efficiency and using optimum transformation efficiency we can find out the insert size.

You might be interested in
Rafel knows that lessons learned is a valuable aid to future projects. When should he and his team address
Arada [10]

Answer: Create lessons learned at the end of the project.

Explanation:

Lessons learned are the experiences that are gotten from a project which should be taken into account for the future projects. Lesson learned are created at the end of the project.

The main objective of the lessons learned is that they show both the positive experience and the negative experience of a project and this will help the future projects that will be undertaken.

5 0
2 years ago
A well-insulated, rigid tank has a volume of 1 m3and is initially evacuated. A valve is opened,and the surrounding air enters at
DiKsa [7]

Answer:

0.5 kW

Explanation:

The given parameters are;

Volume of tank = 1 m³

Pressure of air entering tank = 1 bar

Temperature of air = 27°C = 300.15 K

Temperature after heating  = 477 °C = 750.15 K

V₂ = 1 m³

P₁V₁/T₁ = P₂V₂/T₂

P₁ = P₂

V₁ = T₁×V₂/T₂ = 300.15 * 1 /750.15 = 0.4 m³

dQ = m \times c_p \times (T_2 -T_1)

For ideal gas, c_p = 5/2×R = 5/2*0.287 = 0.7175 kJ

PV = NKT

N = PV/(KT) = 100000×1/(750.15×1.38×10⁻²³)

N = 9.66×10²⁴

Number of moles of air = 9.66×10²⁴/(6.02×10²³) = 16.05 moles

The average mass of one mole of air = 28.8 g

Therefore, the total mass = 28.8*16.05 = 462.135 g = 0.46 kg

∴ dQ = 0.46*0.7175*(750.15 - 300.15) = 149.211 kJ

The power input required = The rate of heat transfer = 149.211/(60*5)

The power input required = 0.49737 kW ≈ 0.5 kW.

3 0
3 years ago
2) The switch in the circuit below has been closed a long time. At t=0, it is opened.
saul85 [17]

Answer:

  il(t) = e^(-100t)

Explanation:

The current from the source when the switch is closed is the current through an equivalent load of 15 + 50║50 = 15+25 = 40 ohms. That is, it is 80/40 = 2 amperes. That current is split evenly between the two parallel 50-ohm resistors, so the initial inductor current is 2/2 = 1 ampere.

The time constant is L/R = 0.20/20 = 0.01 seconds. Then the decaying current is described by ...

  il(t) = e^(-t/.01)

  il(t) = e^(-100t) . . . amperes

8 0
2 years ago
I need ideas for what to build because I have some spare wood.
Misha Larkins [42]

Answer:

small guitar with no strings?

Explanation:

it would be fun to make i think

6 0
3 years ago
An incandescent light bulb can be regarded as a resistor. If its power output is 100W, calculate the resistance of the light bul
stira [4]

Answer:121\ \Omega

0.909\ A

Explanation:

Given

Power P=100\ W

Voltage applied V=110\ V

Resistance of the bulb is given by

P=\frac{V^2}{R}

100=\frac{110^2}{R}

R=\frac{12100}{100}

R=121\ \Omega

Current drawn by the Power source is given by

P=V\cdot I

I=\frac{P}{V}

I=\frac{100}{110}

I=0.909\ A

8 0
3 years ago
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