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viva [34]
3 years ago
13

You don't know which insert you have, and the inserts are different sizes, meaning the amount needed for a 1:3 ratio is differen

t. What do you do? Why? (assume there are enough materials for more than one reaction per person).
Engineering
1 answer:
VashaNatasha [74]3 years ago
6 0

Answer:

Explanation:

For ligation process the 1:3 vector to insert ratio is the good to utilize . By considering that we can take 1 ratio of vector and 3 ratio of insert ( consider different insert size ) and take 10 different vials of ligation ( each calculated using different insert size from low to high ) and plot a graph for transformation efficiency and using optimum transformation efficiency we can find out the insert size.

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A belt drive was designed to transmit the power of P=7.5 kW with the velocity v=10m/s. The tensile load of the tight side is twi
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Answer:

F₁ = 1500 N

F₂ = 750 N

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Explanation:

Given :

Power transmission, P = 7.5 kW

                                      = 7.5 x 1000 W

                                      = 7500 W

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Now we know from power transmission equation

P = ( F₁ - F₂ ) x V

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        F₁ = 2 x 750

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Therefore we know,

F_{max} = 3 x F_{e}

where F_{e} is centrifugal force

 F_{e} = F_{max} / 3

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