1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
viva [34]
3 years ago
13

You don't know which insert you have, and the inserts are different sizes, meaning the amount needed for a 1:3 ratio is differen

t. What do you do? Why? (assume there are enough materials for more than one reaction per person).
Engineering
1 answer:
VashaNatasha [74]3 years ago
6 0

Answer:

Explanation:

For ligation process the 1:3 vector to insert ratio is the good to utilize . By considering that we can take 1 ratio of vector and 3 ratio of insert ( consider different insert size ) and take 10 different vials of ligation ( each calculated using different insert size from low to high ) and plot a graph for transformation efficiency and using optimum transformation efficiency we can find out the insert size.

You might be interested in
Recommend the types of engineers needed to collaborate on a city project to build a skateboard park near protected wetlands.
4vir4ik [10]

Answer:

A civil engineer.

Explanation:

Civil engineering is the science that deals with the design, creation and maintenance of constructions for civil use on the earth's surface. Thus, this specialty seeks to adapt soils to the needs of life in society, creating buildings, bridges, and all other constructions adapted to civil life, while taking care of the correct use of soils and the correct distribution of spaces and resources. to be used for such constructions.

4 0
2 years ago
How does java achieve portable
sergejj [24]

Answer:

Java is called portable because you can compile a java code which will spew out a byte-code, and then you run that code with Java Virtual Machine. Java Virtual Machine is like an interpreter, which reads the compiled byte-code and runs it. So first of all, you need to install the JVM on the system you want.

Explanation:

5 0
3 years ago
By adding "-once", one can form the noun form of the word "organize" is that true or false?​
denpristay [2]

Answer:

<h2>False </h2>

Explanation:

The noun form of organize is just adding letter r

7 0
2 years ago
Read 2 more answers
For powder compaction using a single-action punch, derive an expression for the distribution of axial pressure within a die of r
Zielflug [23.3K]

Answer:

a) 2∪p/lb (l+b)dH

b) po exp( 4∪x/l)

Explanation:

please check the attachment for proper explanation and proper sign notations thanks.

3 0
3 years ago
A six-lane freeway (three lanes in each direction) in rolling terrain has 10-ft lanes and obstructions 5 ft from the right edge.
Anon25 [30]
That is too hard but u got that cuz i believe in you!!!
3 0
3 years ago
Other questions:
  • Define a public static method named s2f that takes two String arguments, the name of a file and some text. The method creates th
    5·1 answer
  • A soil has the following Green-Ampt parameters Effective porosity 0.400 Initial volumetric moisture content-15% Hydraulic Conduc
    6·1 answer
  • Kjhwe ,kenwif ujwfeowlwfwfwfw...
    14·2 answers
  • What is the difference between a single-model production line and a mixed-model production line?
    8·1 answer
  • A 1000 KVA three phase transformer has a secondary voltage of 208/120. What is the secondary full load amperage?
    9·1 answer
  • The velocity of a point mass that moves along the s-axis is given by s' = 40 - 3t^2 m/s, where t is in seconds. Find displacemen
    7·1 answer
  • An inventor tries to sell you his new heat engine that takes in 40 J of heat at 87°C on each cycle, expels 30 J at 27°C, and doe
    14·1 answer
  • The water behind Hoover Dam in Nevada is 221 m higher than the Colorado River below it. At what rate must water pass through the
    6·1 answer
  • An MRI technician moves his hand from a region of very low magnetic field strength into an MRI scanner’s 2.00 T field with his f
    5·1 answer
  • Reverse masking forms a soft edge on the panel.
    5·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!