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ladessa [460]
3 years ago
7

Penicillin is hydrolyzed and thereby rendered inactive by penicillinase, an enzyme present in some resistant bacteria. The mass

of this enzyme in Staphylococcus aureus is 29.6 kd. The amount of penicillin hydrolyzed in 1 minute in a 10-ml solution containing 10^-9 g of purified penicillinase was measured as a function of the concentration of penicillin. A plot of 1/V_0 versus 1/[S] indicated the V_max=6.8 times^- 10 mol minute^-1. Assuming one active site per enzyme molecule, the turnover number for penicillinase is
(A) 337 s^-1
(B) 337 min^-1
(C) 600 hour^-1
(D) 33 hour^-1 600 s^-1
Chemistry
1 answer:
Arisa [49]3 years ago
4 0

Answer:

The turnover number is the maximum substrate quantitiy converted to product per enzyme and per second. It can be calculate as follows:

k_c = \frac{V_{max}}{[E]} with [E] active enzyme concentration.

In this case we have Vmax an data to calculate [E]

moles of E=\frac{10^{-9}g}{29600 g/mol}=3.38*10^{-14}

[E]=\frac{3.38*10^{-14}}{0.01 L}=3.38*10^{-12} M

Now k_c=\frac{6.8*10^-10}{3.38*10^{-12}}=201.28 min^{-1}

it is not like any option.

If we assume that V_{max} have the non usual units of mol L^{-1} min^{-1} and it is 6.8*10^-10 mol min^{-1}

So we need divide by the moles of E (in place of [E])

Now k_c=\frac{6.8*10^-10}{3.38*10^{-14}}=20128 min^{-1} = 335 s{-1}

(pass from min^{-1} to s^{-1} dividing by 60)

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zlopas [31]
<h3>Answer:</h3>

1.83 × 10⁻⁷ mol Au

<h3>General Formulas and Concepts:</h3>

<u>Math</u>

<u>Pre-Algebra</u>

Order of Operations: BPEMDAS

  1. Brackets
  2. Parenthesis
  3. Exponents
  4. Multiplication
  5. Division
  6. Addition
  7. Subtraction
  • Left to Right

<u>Chemistry</u>

<u>Atomic Structure</u>

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<h3>Explanation:</h3>

<u>Step 1: Define</u>

3.60 × 10⁻⁵ g Au (Gold)

<u>Step 2: Identify Conversions</u>

Molar Mass of Au - 196.97 g/mol

<u>Step 3: Convert</u>

  1. Set up:                              \displaystyle 3.60 \cdot 10^{-5} \ g \ Au(\frac{1 \ mol \ Au}{196.97 \ g \ Au})
  2. Multiply:                            \displaystyle 1.82769 \cdot 10^{-7} \ mol \ Au

<u>Step 4: Check</u>

<em>Follow sig fig rules and round. We are given 3 sig figs.</em>

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4 0
3 years ago
What mass of sodium chloride is needed to prepare 200 ml of a 0.1 M solution
Nikolay [14]

Answer:

C. 1.17 grams

Explanation:

  • The molarity is the no. of moles of solute in a 1.0 L of the solution.

<em>M = (mass/molar mass)solute x (1000/ V)</em>

M = 0.1 M, mass = ??? g, molar mass of NaCl = 58.44 g/mol, V = 200.0 mL.

∴ mass of NaCl = (M)(molar mass)(V)/1000 = (0.1 M)(58.44 g/mol)(200.0 mL)/1000 = 1.168 g ≅ 1.17 g.

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Answer:

C. That atoms made up the smallest form of matter

Explanation:

The crux of the Dalton's atomic theory is that atoms are the smallest form of matter. He propositioned that atoms is an indivisible particle and beyond an atom, no form of matter exists.

Series of discoveries through time have greatly shaped the Dalton's atomic theory. The discovery of cathode rays by J.J Thomson in 1897 opened up the atom. Atoms were now seen to be made up of some negatively charged particles. Ernest Rutherford through his gold foil experiment proposed the nuclear model of the atom.

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Why is water called "the universal solvent" and why is this important for living things?
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Answer:

Diasteriomers

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Constitutional isomers differ only in atom to atom connectivity while stereoisomers differ in arrangement of atoms in space. Stereo isomers differ in physical and chemical properties of the compounds.

When stereo isomers are non-superimpossible mirror images of each other, they are called enantiomers. Enantiomers have the same chemical and physical properties and differ only in their reaction with chiral substances.

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