You wish to prepare a buffer consisting of acetic acid and sodium acetate with a total acetic acetate plus acetate concentration
of 250 mm and a ph of 5. what concentrations of acetic acid and sodium acetate should you use
1 answer:
<u>Ans: Acetic acid = 90.3 mM and Sodium acetate = 160 mM</u>
Given:
Acetic Acid/Sodium Acetate buffer of pH = 5.0
Let HA = acetic acid
A- = sodium acetate
Total concentration [HA] + [A-] = 250 mM ------(1)
pKa(acetic acid) = 4.75
Based on Henderson-Hasselbalch equation
pH = pKa + log[A-]/[HA]
[A-]/[HA] = 10^(pH-pKa) = 10^(5-4.75) = 10^0.25 = 1.77
[A-] = 1.77[HA] -----(2)
From (1) and (2)
[HA] + 1.77[HA] = 250 mM
[HA] = 250/2.77 = 90.25 mM
[A-] = 1.77(90.25) = 159.74 mM
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