<span>1s^22s^22p^63s^1 i think</span>
I hope this helps answer some of your questions.
Chemical reaction is given as :

Here, 2 moles of water gives 2 moles of hydrogen and one mole of oxygen.
Mass of hydrogen = 28.0 g (given)
Mass of oxygen = 224.0 g (given)
Number of moles = 
Thus, number of moles of hydrogen = 
= 14 moles of hydrogen
Number of moles of oxygen= 
= 7 moles of oxygen
Now, to find the amount of water:

= 252 g of water.
Thus, amount of water is 252 g (involved in the process).
Answer:
The protein has 4 subunits: 2 subunits of 90 kDa, 1 subunit of 160 kDa and 1 subunit of 60 kDa
Explanation:
In gel electrophoresis, the SDS agent produces denaturation of the protein and confers negative charge, so the protein subunits can migrate according to their masses. It produces dissociation of the protein in its subunits but it cannot disrupt disulphyde bridges (S-S) that can bond subunits together.
From the data, with SDS we observe 3 bands ⇒ 180 kDa + 160 kDa + 60 kDa
The addition of dithiotreitol (DTT), a reducing agent, produces the disruption of disulphyde bridges. From the data:
With DTT ⇒ 160 kDa + 90 kDa + 60 kDa
We observe that 160 kDa and 60 kDa subunits are conserved (they are the same as with SDS only), but 180 kDa subunit is missing and in its place appears a band of 90 kDa - a half 180 kDa.
So, the band at 180 kDa is composed by two subunits bonded by a disulphyde bridge.
Therefore, the composition of the protein is: <em>1 subunit of 160 kDa, 2 subunits of 90 kDa and 1 subunit of 60 kDa</em>.