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ValentinkaMS [17]
3 years ago
14

Imagine you followed all steps correctly, with multiple attempts, and found that the purity of recombinant DHFR isolated through

IMAC was not good enough. Hypothesize two changes to the procedure or experimental design that you could consider trying next to increase the purity of our DHFR sample and why you anticipate each to increase protein purity. (5pt)
Chemistry
1 answer:
Crank3 years ago
7 0

Answer:

fcgvgbjbbbh,bbb bbnnnnmnbb nbmm   m  cccg   n mm

Explanation:

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For the following reaction, 4.31 grams of iron are mixed with excess oxygen gas . The reaction yields 5.17 grams of iron(II) oxi
natka813 [3]

<u>Answer:</u> The theoretical yield of iron (II) oxide is 5.53g and percent yield of the reaction is 93.49 %

<u>Explanation:</u>

To calculate the number of moles, we use the equation:

\text{Number of moles}=\frac{\text{Given mass}}{\text{Molar mass}}       ....(1)

  • <u>For Iron:</u>

Given mass of iron = 4.31 g

Molar mass of iron = 53.85 g/mol

Putting values in above equation, we get:  

\text{Moles of iron}=\frac{4.31g}{53.85g/mol}=0.0771mol

For the given chemical reaction:

2Fe(s)+O_2(g)\rightarrow 2FeO(s)

By Stoichiometry of the reaction:

2 moles of iron produces 2 moles of iron (ii) oxide.

So, 0.0771 moles of iron will produce = \frac{2}{2}\times 0.0771=0.0771mol of iron (ii) oxide

Now, calculating the theoretical yield of iron (ii) oxide using equation 1, we get:

Moles of of iron (II) oxide = 0.0771 moles

Molar mass of iron (II) oxide = 71.844 g/mol

Putting values in equation 1, we get:  

0.0771mol=\frac{\text{Theoretical yield of iron(ii) oxide}}{71.844g/mol}=5.53g

To calculate the percentage yield of iron (ii) oxide, we use the equation:

\%\text{ yield}=\frac{\text{Experimental yield}}{\text{Theoretical yield}}\times 100

Experimental yield of iron (ii) oxide = 5.17 g

Theoretical yield of iron (ii) oxide = 5.53 g

Putting values in above equation, we get:

\%\text{ yield of iron (ii) oxide}=\frac{5.17g}{5.53g}\times 100\\\\\% \text{yield of iron (ii) oxide}=93.49\%

Hence, the theoretical yield of iron (II) oxide is 5.53g and percent yield of the reaction is 93.49 %

7 0
2 years ago
Consider the reaction pathway graph below.
Alex17521 [72]
Hello!

The reaction that the graph represents is A. Exothermic because Hrxn=-167 kJ

To calculate Hrxn we apply the following equation:

Hrxn=Hproducts-Hreagents=-625kJ-(-458kJ)=-167kJ

Looking at the graph, and at the result of the calculations, we can see that the enthalpy of the products is lower than the enthalpy of the reagents, because the sign is negative. That means that the reaction releases energy in the form of heat and that the reaction is exothermic.

Have a nice day!
6 0
3 years ago
Can someone tell me how to differentiate if a given salt is acidic, basic, or neutral?​
satela [25.4K]

\bold{\huge{\underline{ Solution }}}

<h3><u>Basic </u><u>Characteristic </u><u>of </u><u>acids </u></h3>

  • Acids are sour in taste
  • Acid turns blue litmus paper or solution into red litmus paper or solution
  • Acids are good conductor of electricity because it dissociate into cation in aqueous solution
  • Acids classified into edible acids and non edible acids. Non edible acids are very hazardous
  • Generally, All acids are soluble in water
  • Acids have PH smaller than 7

<u>Arrhenius </u><u>definition </u><u>of </u><u>acids </u><u>:</u><u>-</u>

According to Arrehinus,

  • Acids are those substances which when dissolve in water given H positive ions . Then, this hydrogen ions combine with water to form H30 + ions

<u>Second </u><u>definition </u><u>of </u><u>Acids </u><u>was </u><u>given </u><u>by </u><u>Bonsted </u><u>Lowry </u><u>:</u><u>-</u>

According to Bonsted Lowry

  • Acids are the proton donors that is when acids dissociate into water gives hydrogen ions that is H+ ions

<u>3rd </u><u>definition </u><u>was </u><u>given </u><u>by </u><u>Lewis </u>

According to Lewis

  • Acids are those substances which have the ability to accept a pair of electrons .

Example of Acids

  • HNO3 :- Nitric acid
  • H2SO4 :- Sulfuric acid
  • HCl :- Hydrochloric acid

<h3><u>Basic </u><u>characterists </u><u>of </u><u>bases </u></h3>

  • Bases are bitter in taste
  • Bases turns red litmus paper into blue litmus paper or solution
  • Bases are also good conductor of electricity because on dissociation it produces anion in aqueous solution
  • Bases are also good conductor of electricity
  • When bases are soluble in water then they are known as alkaline base
  • Bases have PH greater than 7

<u>Arrehinus definition of bases :-</u>

According to Arrehinus ,

  • Bases are those substances which when dissolve in water produce OH negative ions that is hydroxide ions

<u>Bonsted Lowry definition </u>

According to Bonsted Lowry

  • Bases are the proton donors as they produce OH negative ions in dissociation in aqueous solution

<u>Lewis </u><u>definition </u>

According to Lewis

  • Acids are those substances which have the ability to lose electrons that is they are electron donors.

Example of bases

  • Ca(OH)2 :- Calcium hydroxide
  • NaOH :- Sodium hydroxide
  • KOH :- Potassium hydroxide

[ Note :- There are so many Lewis acids and bases but they are not Arrhenius or Lowry acids or bases ]

<h3><u>Basic </u><u>characteristic </u><u>of </u><u>salt </u></h3>

  • Salts are the ionic compounds which are composed of acids and bases that cation and anion
  • Salts are generally found in oceans and seas in the forms of crystals
  • As they are composed of acids and bases so they are neutral in nature but the salt of strong acid or weak base is acidic in nature or vice versa
  • Salts are also good conductor of electricity as they form ionic bond
  • Generally, All salts are soluble in water.
  • The PH of common Salt is 7

Example of salts

  • NH4Cl :- Ammonium chloride
  • CuSO4 :- Copper sulphate
  • NaCl :- Sodium chloride
6 0
2 years ago
What is the principle feature of a metallic bond?
Kobotan [32]
Electrons are free to move throughout metal substance, shared throughout so electricity and heat are conducted well
4 0
3 years ago
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What are two characteristics of a good scientific investigation
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It's observation and a hypothesis.
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