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stellarik [79]
3 years ago
10

The 10x SDS gel electrophoresis buffer contains 250mM Tris HCl, 1.92M Glycine, and 1% (w/v) SDS. Buffers are always used at 1x c

oncentration in the lab (unless specified otherwise in the protocol), so you will have to dilute the 10x buffer to 1x before use. What is the concentration of Tris and SDS in the 1x buffer
Chemistry
1 answer:
olchik [2.2K]3 years ago
7 0

Answer:

25 mM Tris HCl and 0.1% w/v SDS

Explanation:

A <em>10X solution</em> is ten times more concentrated than a <em>1X solution</em>. The stock solution is generally more concentrated (10X) and for its use, a dilution is required. Thus, to prepare a buffer 1X from a 10X buffer, you have to perform a dilution in a factor of 10 (1 volume of 10X solution is taken and mixed with 9 volumes of water). In consequence, all the concentrations of the components are diluted 10 times. To calculate the final concentration of each component in the 1X solution, we simply divide the concentration into 10:

(250 mM Tris HCl)/10 = 25 mM Tris HCl

(1.92 M glycine)/10 = 0.192 M glycine

(1% w/v SDS)/10 = 0.1% w/v SDS

Therefore the final concentrations of Tris and SDS are 25 mM and 0.1% w/v, respectively.

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Refer to the Chemical Compounds table above. How many total elements are in<br> Ethanol?
Korolek [52]

Answer:

3 element i.e carbon (C), hydrogen (H) and oxygen.

Explanation:

The following data were obtained from the question:

Subtance >>>>>>>> Chemical Formula

Glucose >>>>>>>>> C₆H₁₂O₆

Methane >>>>>>>> CH₄

Ethanol >>>>>>>>> C₂H₅OH

Hydrogen peroxide >> H₂O₂

From the above table, we can see that ethanol (C₂H₅OH) contains carbon (C), hydrogen (H) and oxygen

Therefore, the total number of elements present in ethanol, C₂H₅OH is 3.

8 0
3 years ago
How many bromine atoms are present in 39.0 g of ch2br2?
Mandarinka [93]
Answer is: there is  2,69·10²³ atoms of bromine.
m(CH₂Br₂) = 39,0 g.
n(CH₂Br₂) = m(CH₂Br₂) ÷ M(CH₂Br₂).
n(CH₂Br₂) = 39 g ÷ 173,83 g/mol.
n(CH₂Br₂) = 0,224 mol.
In one molecule of CH₂Br₂, there is two bromine atoms, so:
n(CH₂Br₂) : n(Br) = 1 : 2.
n(Br) = 0,448 mol.
N(Br) = n(Br) · Na.
N(Br) = 0,448 mol · 6,022·10²³ 1/mol.
n(Br) = 2,69·10²³.
7 0
3 years ago
Chemical formula for Potassium Hydrogen Nitrate
My name is Ann [436]

Answer:

KNO3

Explanation:

8 0
3 years ago
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Answer:

b 13kg/m³ is the answer to this question

8 0
3 years ago
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Care to help me guys please​
earnstyle [38]

Answer:

A. Na₂SO₄ and HCl

C. Polar solutes are soluble in polar solvents but are insoluble in non-polar solvents Non-polar solutes are insoluble in polar solvents but are are soluble in non-polar solvents

Ionic solutes are soluble in polar solvents but are insoluble in non-polar solvents.

Like dissolves like simply means that molecules of substances having similar chemical properties dissolve in each other

Explanation:

A. Ionic substances like Na₂SO₄ are composed of charged particles called ions. These ions are either positively charged or negatively charged, therefore, they are attracted to substances of opposite charges. Also, polar molecules like HCl contains two oppositely charged ends. A polar solvent consists of molecules with two oppositely charged ends, therefore, ionic substances as well polar substances dissolve in them according to the concept of like dissolves like.

Gasoline being non-polar will only dissolve in like substances, polar solvents.

C. Polar solutes are soluble in polar solvents but are insoluble in non-polar solvents Non-polar solutes are insoluble in polar solvents but are are soluble in non-polar solvents

Ionic solutes are soluble in polar solvents but are insoluble in non-polar solvents.

The statement "Like dissolves like" simply means that molecules of substances having similar chemical properties dissolve in each other. For example gasoline, a non-polar substance will dissolve only in a non-polar solvent like kerosene. Also, HCl, a polar molecule will dissolve in a polar solvent like water.

7 0
2 years ago
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