A locating agent is needed in an experiment to separate amino acids by chromatography as it helps to analyze colorless substances on paper.
<h3>What is chromatography?</h3>
Chromatography is an analytical method frequently used to separate a chemical mixture into its components, allowing for in-depth analysis of each component.
There are many different types of chromatography including liquid, gas, ion-exchange, and affinity chromatography, but they all use the same basic principle.
<h3>What is the use of a locating agent in Chromatography?</h3>
In paper chromatography, colorless compounds are examined using a locating agent.
It is a chemical that combines with colorless substances to produce colorful compounds that are easy to see for analysis. Ninhydrin spray is an example of a locating agent of this type.
Thus, a locating agent is used in an experiment to separate amino acids by chromatography as it helps in determining colorless substances on paper.
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Answer: Moles of hydrogen required are 4.57 moles to make 146.6 grams of methane, 
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Explanation:
Given: Mass of methane = 146.6 g
As moles is the mass of a substance divided by its molar mass. So, moles of methane (molar mass = 16.04 g/mol) are calculated as follows.
The given reaction equation is as follows.
This shows that 2 moles of hydrogen gives 1 mole of methane. Hence, moles of hydrogen required to form 9.14 moles of methane is as follows.
Thus, we can conclude that moles of hydrogen required are 4.57 moles to make 146.6 grams of methane, .
Answer: one molecule of O2.
Explanation: sweet i just took a guess but I believe that if 3 o2 molecules - 2 h2 molecules I think that its just basic maths and it is C because 3-2 = 1 and its o2 remaining, sorry if I’m wrong.
