Hey there!
To find this, you should:
1) multiply the percentage (but in a decimal form) of each isotope by the mass.
2) add all your masses together
3) make sure to check for significant figures and you’re done!
Hope this helps :)
The colored light emitted is energy and in order to emit energy the element should first obtain energy. The energy absorbed by the substance can be in the form of radiation, heat or electricity. Hope this answers the question. Have a nice day.
Solution :
For the reaction :

we have
![$Ka = \frac{[\text{Tris}^- \times H_3O]}{\text{Tris}^+}$](https://tex.z-dn.net/?f=%24Ka%20%3D%20%5Cfrac%7B%5B%5Ctext%7BTris%7D%5E-%20%5Ctimes%20H_3O%5D%7D%7B%5Ctext%7BTris%7D%5E%2B%7D%24)


Clearing
, we have 
So to reach
, one must have the
concentration of the :
![$\text{[OH}^-]=10^{-pOH} = 6.31 \times 10^{-7} \text{ moles of base}$](https://tex.z-dn.net/?f=%24%5Ctext%7B%5BOH%7D%5E-%5D%3D10%5E%7B-pOH%7D%20%3D%206.31%20%5Ctimes%2010%5E%7B-7%7D%20%5Ctext%7B%20moles%20of%20base%7D%24)
So we can add enough of 1 M NaOH in order to neutralize the acid that is calculated above and also adding the calculated base.


Volume NaOH 
Tris mass 
Now to prepare the said solution we must mix:
gauge to 1000 mL with water.