<span>The following is the role of NaCl and EDTA in DNA isolation, hope it helps:
NaCl provides Na+ ions that will block negative charge from phosphates on DNA.
Negatively charged phosphates on DNA cause molecules to repel each other. The Na+ ions will form an ionic bond with the negatively charged phosphates on the DNA, neutralizing the negative charges and allowing the DNA molecules to come together.</span>
Answer is: pKa for the
monoprotic acid is 5.<span>
Chemical reaction: HA(aq) </span>⇄ A⁻(aq) + H⁺<span>(aq).
c(monoprotic acid) = 0.100 M.
pH = 3.00.
[A</span>⁻] = [H⁺] = 10∧(-3).<span>
[A</span>⁻]
= [H⁺] = 0.001 M; equilibrium concentration.<span>
[HA] = 0.1 M - 0.001 M.
[HA] = 0.099 M.
Ka = [A</span>⁻]·[H⁺] / [HA].<span>
Ka = (0.001 M)² / 0.099 M.
Ka = 0.00001 M = 1.0·10</span>⁻⁵ M.
<span>pKa = -logKa = 4.99.
</span>
Answer:
chemical means
Explanation:
Compounds can be separated by chemical means physical means boiling pressure
