Answer:
Eqv Pt pH = 8.73
Explanation:
HOAc + NaOH => NaOAc + H₂O
50ml(0.10M HOAc) + 50ml(0.10M NaOH) => 100ml(0.05M NaOAc) + H₂O
For neutralized system, 100ml of 0.05M NaOAc remains
NaOAc => Na⁺ + OAc⁻
Na⁺ + H₂O => No Rxn
OAc⁻ + H₂O => HOAc + OH⁻
C(i) 0.05M ----- 0M 0M
ΔC -x ----- +x +x
C(f) 0.05-x
≅ 0.05M ----- x x
Kb = Kw/Ka = [HOAc][OH⁻]/[OAc⁻] = 1 X 10⁻¹⁴/1.7 X 10⁻⁵ = (x)(x)/(0.05M)
=> x = [OH⁻] = SqrRt(0.05 x 10⁻¹⁴/1.7 x 10⁻⁵) = 5.42 x 10⁻⁶M
=> pOH = -log[OH⁻] = -log(5.42 x 10⁻⁶) = 5.27
pH + pOH = 14 => pH = 14 - pOH = 14 - 5.27 = 8.73 Eqv Pt pH
Explanation:
DNA is composed of large amount of amino acids and have a lot of amide groups .
Therefore , in the acidic or in basic medium , the DNA molecules is either dissociated or dis stabilized via protonation or de protonation .
<u>The double helix structure of the DNA may break or get dis ordered .</u>
But , from the question , In sodium phosphate buffer at pH = 7.0 , is the neutral condition , hence , the DNA remains unchanged , because of the neutral medium .
And in pure water ,
The DNA molecule remains intact as , in pure water , there are no ions .