1) divide each percentage by the relative atomic mass of the element
2) divide all results by the smallest number
3)multiply by a whole number to get the simplest whole number ratio (if necessary)
that is to say:
Na S O
32.37÷23 22.58÷32 45.05÷16
= 1.407 = 0.7056 = 2.816 (to 4 significant figures)
the smallest number here is 0.7056 so:
1.407÷0.7056 0.7056÷0.7056 2.816÷0.7056
=1.99 approx.2 = 1 3.99 approx. 4
here there is no need to carry out step 3 as ratio obtained is already a simplest whole number ratio
so empirical formula is: Na₂SO₄
Mercury, Venus, Earth,Mars
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Agar is used to assist establish an anaerobic environment that promotes nitrate reduction.
Nitrate Reduction test:
- The nitrate in the broth is converted to nitrite by organisms that can produce the nitrate reductase enzyme, which can then be further converted to nitric oxide, nitrous oxide, or nitrogen.
- Anaerobic respiration and denitrification are two processes that can convert nitrate to a variety of compounds.
- While denitrification only reduces nitrate to molecular nitrogen, anaerobic respiration employs nitrate as the bacterium's final electron acceptor, reducing it to a range of chemicals.
- The nitrate reduction test is based on the detection of nitrite and its capacity to produce a red precipitate (prontosil), which is a water-soluble azo dye, when it combines with sulfanilic acid to create a complex (nitrite-sulfanilic acid).
Learn more about the Nitrate reduction test with the help of the given link:
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