The shape of the molecule will determine the direction of each of the individual bond dipoles, and thus, will always play a role in determining the polarity of the molecule as a whole.
Absorbance measures the ability of the substance to absorb light at a specific wavelength.
Absorbance is also equal to the product of molar absorptivity, path length and molar concentration.
The mathematical expression is given as:
(1)
where, A = absorbance
= molar absorptivity
l = path length
c = molar concentration.
The above formula is said to Beer's Law.
Absorptivity of protein x = 
Path length = 1 cm
Molar concentration = 
Put the values in formula (1)
= 
Thus, absorbance at 280 nm =
Answer:
1,2 dichlorobenzene was used as the solvent for the diels alder reaction: <em>because the co elimination part of the reaction needs high temp and a high boiling point solvent such as 1,2 dichlorobenzene</em>
Explanation:
Diels-Alder Reaction is a useful synthetic tool to prepare cyclohexane rings. It is a process, which occurs in a single step that consists of a cyclic redistribution of its electrons. The two reagents are bond together through a cyclic transition state in which the two new C-C bonds are formed at the same time. For this to occur, most of the time, it is necessary a high temperature and high-pressure conditions. Since 1,2 dichlorobenzene has a boiling point of 180ºC is a good solvent for this type of reactions.
