Question

8. What was the original concentration in the BHL sample, if the dilution is 1:500 and the concentration 0.07 mg/ml

Answer 1

Answer:

The original concentration is "35 mg/ml".

Explanation:

According to the question,

The solution is diluted,

= 1:50

The initial volume,

V1 = 1 ml

Final concentration,

= 0.07 mg

then,

The final volume,

V2 = 500 ml

As we know,

⇒ $V_1N_1=V_2N_2$

or,

⇒ $N_1=\frac{V_2N_2}{V_1}$

On substituting the values, we get

⇒       $=\frac{500\times 0.07}{1}$

⇒       $=\frac{35}{1}$

⇒       $=35 \ mg/ml$