well if you don't what to call him out the like just kinda explain it to him nicely and if you don't want to do that the just inore him do what's best for you

Explanation:

do what you thinks right

4 0

3 years ago

You are given stocks of 4 M NaCl, 40% Glucose, and 1M Tris-HCl (pH 8.5). You need to make 400 ml of a buffer containing 0.5 M Na

OLga [1]

Answer:

50mL of 4M NaCl, 80mL of 40% glucose, 20mL of 1M Tris-HCl (pH 8.5) and 250mL of water.

Explanation:

To make 400mL containing 0.5M NaCl you need to add:

4M / 0.5M = 8 (dilution 1/8). 400mL / 8 = 50 mL of 4M NaCl.

Glucose 8% you need to add:

40% / 8% = 5 (dilution 1/5). 400mL / 5 = 80 mL of 40% glucose 

Buffer 50mM you need to add:

1000mM / 50mM = 20 (dilution 1/20). 400mL / 20 = 20mL of 1M Tris-HCl (pH 8.5)

The resting volume: 400mL - 50mL of 4M NaCl - 80mL of 40% glucose - 20mL of 1M Tris-HCl (pH 8.5) = 250 mL must be completed with water.

Thus, to make the solution you need: 50mL of 4M NaCl, 80mL of 40% glucose, 20mL of 1M Tris-HCl (pH 8.5) and 250mL of water.

I hope it helps!

8 0

3 years ago