1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Solnce55 [7]
2 years ago
13

For the wild type (unmutated) enzyme, you measure a rate of p-nitrophenol release by the change in absorbance at 405 nm (for the

slower phase only) of 0.216 (in a cuvette with a pathlength of 1 cm) over the course of two minutes. Use the Beer-Lambert Law to calculate the concentration of product released over this time; the extinction coefficient of p-nitrophenol at 405 nm is 18,000 M-1 cm-1.
Chemistry
1 answer:
Aleks04 [339]2 years ago
4 0

Answer:

1.2x10⁻⁵M = Concentration of the product released

Explanation:

Lambert-Beer's law states the absorbance of a solution is directly proportional to its concentration. The equation is:

A = E*b*C

<em>Where A is the absotbance of the solution: 0.216</em>

<em>E is the extinction coefficient = 18000M⁻¹cm⁻¹</em>

<em>b is patelength = 1cm</em>

<em>C is concentration of the solution</em>

<em />

Replacing:

0.216 = 18000M⁻¹cm⁻¹*1cm*C

<h3>1.2x10⁻⁵M = Concentration of the product released</h3>
You might be interested in
Ammonia and hydrogen fluoride both have unusually high boiling points due to
nexus9112 [7]

Answer:

C. hydrogen bonding

Explanation:

Ammonia and hydrogen fluoride are both able to exhibit hydrogen bonding due to containing nitrogen (in ammonia) and fluoride (obviously in hydrogen fluoride). Remember the unique qualities of NOF. :)

5 0
3 years ago
A 8.00 L tank at 26.9 C is filled with 5.53 g of dinitrogen difluoride gas and 17.3 g of sulfur hexafluoride gas. You can assume
pshichka [43]

Answer:

mole fraction of N_2 O = 0.330

mole of fraction SF_4 = 0.669

PRESSURE OF N_2 O = 39127.053 Pa

pressure of SF_4 = 792126.36

Total pressure   = 118253.413 Pa

Explanation:

Given data:

volume of tank 8 L

Weight of dinitrogen difluoride gas 5.53 g

weight of sulphur hexafluoride gas 17.3 g

Amount of N_2 O = \frac{5.53}{14*2 + 16} = 0.1256 mol

amount of SF_4 = \frac{17.3}{32.1 + 19*4} = 0.254 mol

mole fraction of N_2 O = \frac{0.1256}{0.1256 + 0.254} = 0.330

mole of fractionSF_4 = \frac{0.254}{0.1256 + 0.254} = 0.669

PV = nRT

P of N_2 O = \frac{0.1256 *8.31 (273 + 26.9}{0.008} = 39127.053 Pa

mole of SF_4=\frac{0.254 *8.31*(273+26.9)}{.008} = 79126.36 Pa

Total pressure  = 39127.053 + 79126.36 = 118253.413 Pa

6 0
3 years ago
I build a fire and burn 3 logs. Apply the Law of Conservation of Matter
photoshop1234 [79]
Capture all of the smoke and weight it. it will weigh exactly the same before and after you burn it but will just be CO2 and H2O gas.
7 0
3 years ago
26 g of zinc combines with 12.8 g of sulfur. What is the empirical formula of zinc sulfide?
Alisiya [41]
Moles of Zn: 26 / 65 = 0.4
Moles of S: 12.8 / 32 = 0.4

Molar ratio of Zn : S = 1 : 1
Empircal formula: ZnS

The answer is C
7 0
3 years ago
The liter is used to measure _____.<br><br> weight<br> mass<br> volume<br> length
Ket [755]

the answer to your question is

volume

4 0
3 years ago
Read 2 more answers
Other questions:
  • What type of bond forms when electrons are transferred (lose or gain) from one atom to another?
    15·1 answer
  • What family are the elements carbon, germanium and lead in?
    13·2 answers
  • Determine whether each statement is a description of a physical property or a chemical property.
    14·1 answer
  • Which metals have similar chemical property’s? Check all that apply.
    14·2 answers
  • How does the Miller Urey experiment
    7·1 answer
  • A gas expands from 2.5 L to 8.6 L at a constant temperature. If the initial pressure is 1.6 atm, what is the final pressure?
    9·1 answer
  • Help ASAP<br> How many moles of O2 are in 7.23 mol P
    13·1 answer
  • What do water, air and rocks have in common
    10·1 answer
  • For each balanced reaction, indicate the total number of molecules in the table below.
    5·1 answer
  • How do I know if something is an acid so I can avoid eating it?
    7·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!