Answer:
light energy to convert carbon dioxide and water into glucose and oxygen gas. Each molecule of glucose essentially “stores” up to 38 molecules of ATP which can be broken down and used during other cellular reactions.
Explanation:
Answer:
431.38 mg protein / mL
Explanation:
This is an example of the <em>Kjeldahl method</em>, for nitrogen determination. All nitrogen atoms in the protein were converted to NH₃ which then reacted with a <u>known excess of HCl</u>. This excess was later quantified via titration with NaOH.
First we calculate the <u>total amount of H⁺ moles from HCl</u>:
- 0.0388 M HCl * 10.00 mL = 0.388 mmol H⁺
Now we calculate the <u>excess moles of H⁺</u> (the moles that didn't react with NH₃ from the protein), from the <u>titration with NaOH</u>:
- HCl + NaOH → H₂O + Na⁺ + Cl⁻
- 0.0196 M * 3.83 mL = 0.075068 mmol OH⁻ = 0.0751 mmol H⁺
Now we substract the moles of H⁺ that reacted with NaOH, from the total number of moles, and the result is the <u>moles of H⁺ that reacted with NH₃ from the protein</u>:
- 0.388 mmol H⁺ - 0.0751 mmol H⁺ = 0.313 mmol H⁺ = 0.313 mmol NH₃
With the moles of NH₃ we know the moles of N, then we can <u>calculate the mass of N</u> present in the aliquot:
- 0.313 mmol NH₃ = 0.313 mmol N
- 0.313 mmol N * 14 mg/mmol = 4.382 mg N
From the exercise we're given the concentration of N in the protein, so now we <u>calculate the mass of protein</u>:
- 4.382 mg * 100/15.7 = 27.91 mg protein
Finally we <u>calculate the protein concentration in mg/m</u>L, <em>assuming your question is in 647 μL</em>, we first convert that value into mL:
- 647 μL *
0.647 mL
- 27.91 mg / 0.647 mL = 431.38 mg/mL
Taking credit for a someone else's research or written work
I believe it is type, from what I know.