At an optimum pH of 7.0, there are more molecules per minute in all amounts of substrate thus this pH is ideal for maximum growth. 5. Enzymes function most efficiently at the temperature of a typical cell, which is 37 degrees Celsius. Increases or decreases in temperature can significantly lower the reaction rate.
10 cubic inches
We will use Boyle's law that states that for a fixed amount of an ideal gas kept at a fixed temperature, pressure and volume are inversely proportional.
P1 V1 = P2 V2
Where
P1 is initial pressure = 5 psi
V1 is initial volume = 20 cubic inch
P2 is final pressure = 10 psi
V2 is final volume = unknown
V2 = P1,V1 / P2
V2 = 20 × 5 / 10
V2 = 100/10
V2 = 10 cubic inches
