Question

A DNA sample and a plasmid with the ampR and lacZ(for the enzyme beta-galactosidase) genes are treated with Pstl with the goal of making recombinant DNA molecules. the lacZ gene is placed in the cloning region so that it is disrupted by DNA inserts. Put the remaining steps for this process in the right order. NOT ALL statements will be used! The first step has already been given to you.
step options: -all the fragments have the sticky ends 5'-CTGCAG-3'
-cells containing the recombinant plasmid are grown in a medium with
ampicillin and x-gal(a galactose derivative)
-blue colonies that survive on the culture medium contain the
recombinant plasmid
-all the fragments have identical sticky ends: 5'-TGCA-3'
-colonies containing the recombinant plasmid can be removed from the
culture medium and grown
-DNA ligase joins the sticky ends of the DNA sample and the plasmid
-white colonies that survive on the culture medium are identified as
having the recombinant plasmid
step 1) DNA is treated with restriction enzyme
step 2)
step 3)
step 4)
step 5)
step 6)

Answer 1

Answer:

Explanation:

The main aim of this experiment is to correctly identify the sequential steps needed for making recombinant DNA molecules.

The first step is given as:

DNA is treated with restriction enzyme

The remaining steps in order are:

$\text{-all the fragments have identical sticky ends: 5'-TGCA-3'}$

$\text{-DNA ligase joins the sticky ends of the DNA sample and the plasmid}$

$\text{-cells containing the recombinant plasmid are grown in a medium with}$

$\text{ampicillin and x-gal(a galactose derivative)}$

$\text{-white colonies that survive on the culture medium are identified as}$

$\text{having the recombinant plasmid}$

$\text{-colonies containing the recombinant plasmid can be removed from the}$

$\text{culture medium and grown}$