The bacteria that doesn’t live in extreme conditions is Eubacteria
Whichever has the most mass so probably or trailer SUV
The weight is 45 N, because the three chains hold the sign, and each contributes 15 N.
Notice that the mass would be the weight/acceleration of gravity, m = 45/9.8 kg. But they ask the weight (force, so Newtons)
A=(vf-vi)/t
a=(50-25)/10
a=2.5m/s^2