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HA ⇄ H⁺ + A⁻
so:
![\frac{[H^+][A^-]}{[HA]} = 1.5 x 10^{-5}](https://tex.z-dn.net/?f=%20%5Cfrac%7B%5BH%5E%2B%5D%5BA%5E-%5D%7D%7B%5BHA%5D%7D%20%3D%201.5%20x%2010%5E%7B-5%7D%20%20)
and now:
= 1.5 x 10⁻⁵
x is considered very small compared to 0.15
x² = 2.25 x 10⁻⁶
x = 1.5 x 10⁻³
So [H⁺] = 1.5 x 10⁻³
pH = - log [H⁺] = - log (1.5 x 10⁻³) = 2.83
so:
and now:
x is considered very small compared to 0.15
x² = 2.25 x 10⁻⁶
x = 1.5 x 10⁻³
So [H⁺] = 1.5 x 10⁻³
pH = - log [H⁺] = - log (1.5 x 10⁻³) = 2.83
Answer: The approximate molecular mass of the polypeptide is 856 g/mol
Explanation:
To calculate the concentration of solute, we use the equation for osmotic pressure, which is:
Or,
where,
= osmotic pressure of the solution = 4.19 torr
i = Van't hoff factor = 1 (for non-electrolytes)
Mass of solute (polypeptide) = 0.327 g
Volume of solution = 1.70 L
R = Gas constant =
T = temperature of the solution =
Putting values in above equation, we get:
Hence, the molar mass of the polypeptide is 856 g/mol