Answer:
The protein has 4 subunits: 2 subunits of 90 kDa, 1 subunit of 160 kDa and 1 subunit of 60 kDa
Explanation:
In gel electrophoresis, the SDS agent produces denaturation of the protein and confers negative charge, so the protein subunits can migrate according to their masses. It produces dissociation of the protein in its subunits but it cannot disrupt disulphyde bridges (S-S) that can bond subunits together.
From the data, with SDS we observe 3 bands ⇒ 180 kDa + 160 kDa + 60 kDa
The addition of dithiotreitol (DTT), a reducing agent, produces the disruption of disulphyde bridges. From the data:
With DTT ⇒ 160 kDa + 90 kDa + 60 kDa
We observe that 160 kDa and 60 kDa subunits are conserved (they are the same as with SDS only), but 180 kDa subunit is missing and in its place appears a band of 90 kDa - a half 180 kDa.
So, the band at 180 kDa is composed by two subunits bonded by a disulphyde bridge.
Therefore, the composition of the protein is: <em>1 subunit of 160 kDa, 2 subunits of 90 kDa and 1 subunit of 60 kDa</em>.
Answer:
the volume of CHCI3 = 7.87 ml
the volume of CHBr3 = 12.13 ml
Explanation:
From the given information:
We all know that 1 g/cm^3 = 1 g/ml
The density of boron = 2.34 g/ml
The Volume of the liquid mixture = 20 ml
Recall that:
Density = mass/volume
Mass = Density × Volume
Mass = 2.34 g/ml × 20 ml
Mass = 46.8 g
Suppose the volume of CHCI3 be Y and the Volume of CHBr3 be 20 - Y
Then :
Y (1.492) + 20-Y(2.890) = 46.8
1.492Y + 57.8 - 2.890Y = 46.8
- 1.398 Y = -11
Y = -11/ - 1.398
Y = 7.87 ml
Therefore, the volume of CHCI3 7.87 ml
the volume of CHBr3 = 20 - Y
= 20 - 7.87
= 12.13 ml
These waxes are highly hydrophobic (repellant) of water, so the correct answer would be D) repel.
The wax coating put on the car creates a water repellant sheila that causes the water to bead up on the surface of the wax, rather than directly impacting the paint and surface of the body of the car. This prolongs the life and quality of the appearance of the car.
Answer:
Well they didn't transfer any energy when they weren’t touching and it did t produce any energy if it didn’t move. Since they are on top of each other they are causing momentum on each other creating kinetic energy
Explanation: