Question

Identify the role of all molecular constituents present in a functional polyribosome. Drag the terms on the left to the appropriate blanks on the right to complete the sentences.

Answer 1

A polyribosome (or polysome) is a complex of one mRNA molecule and two or more ribosomes that  form a polypeptide chain during active translation. Since multiple ribosomes can translate  mRNA simultaneously, many polypeptides can be produced simultaneously from a single mRNA.

Role of all molecular constituents present in a functional polyribosome ;

1. mRNA: contains the codon

2. tRNA : contains the anticodon for the mRNA

3. Ribosome (large & small subunits): facilitates interaction between mRNA and tRNA

4. Amino acids:

5. Initation Factors: proteins important in the interaction/binding of the small subunit, mRNA, TRNAmet, and the Shine Dalgarno sequence

7. Elongation Factors: bring charged tRNA's to the A site of the ribosome

8. GTP: provides energy

Ribosome recruitment in eukaryotes occurs when the eukaryotic initiation factor e1F4F and poly(A) binding protein (PABP) recognize 5′-capped mRNAs and recruit the 43S ribosome complex to this site. increase. In addition, the Kozak consensus sequence ACCAUGG is also required for translation initiation. Therefore, at first glance, it seems unlikely that  overlapping sequences will be translated.

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