Using a thin stationary phase supported by an inert backing, thin layer chromatography (TLC) is a chromatographic technique used to separate the components of a mixture.
It can be carried out on an analytical scale to track the development of a reaction or on a preparative scale to purify minute quantities of a chemical. Because of its simplicity, comparatively low cost, great sensitivity, and rapid separation, TLC is an extensively used analytical method. Similar to all chromatography, TLC works on the premise that a chemical will have varying affinities for the mobile and stationary phases, which will influence how quickly it migrates. TLC aims to produce well-defined, well-separated spots.
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