In the preparatory phase of glycolysis, two molecules of ATP are invested and the hexose chain is cleaved into two triose phosphates. During this, the phosphorylation of glucose and its conversion to glyceraldehyde-3-phosphate take place. During this phase, the conversion of glyceraldehyde-3-phosphate to pyruvate and the coupled formation of ATP take place. Because Glucose is split to yield two molecules of D-Glyceraldehyde-3-phosphate, each step in the payoff phase occurs twice per molecule of glucose.
Glyceraldehyde 3-phosphate dehydrogenase Simultaneous oxidation and phosphorylation of G3P produce 1,3-bisphosphoglycerate (1,3-BPG) and nicotine adenine dinucleotide (NADH).
The divalent cation also affected the response of the enzyme from the endosperm and shoots to adenine nucleotides and inorganic pyrophosphate.
This phase is also called the glucose activation phase. In the preparatory phase of glycolysis, two molecules of ATP are invested and the hexose chain is cleaved into two triose phosphates. During this, the phosphorylation of glucose and its conversion to glyceraldehyde-3-phosphate take place. Steps 1, 2, 3, 4, and 5 together are called the preparatory phase.
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