Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula 
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.
The stock concentration of NaCl (5 M ) is as follows:
.
The stock concentration of Imidazole (1 M ) is as follows:
.
Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
The volume<span> of </span>gas<span> because of the </span>increase<span> and decrease in the speed in which the molecules bounce around. ... Boyle's Law states that if temperature stays the same, the </span>amount of<span> space a </span>gas takes up will increase<span> if the </span>pressure<span> decreases. The </span>amount of gas<span> will take up less space if the </span>pressure<span> is increased. this would be the correct answer </span>
climate
Changes in the composition of the atmosphere have caused gradual changes in earth's <u>climate</u> throughout history, causing changes in plant and animal life that contributed to mass extinctions.
The following are some of the reasons:
- UV light
- climate
- pollutants
- hydrofluorocarbons
heat
- The surface of the Earth warms up as sunlight strikes it.
- Surface-emitted infrared light is absorbed in the atmosphere and transformed into heat.
- The temperature close to the surface rises as a result of this heat being trapped in the atmosphere.
<h3>UV light:</h3>
- indirect impacts of climate change on UV radiation from the surface.
- By changing the concentrations of ozone, UV-absorbing tropospheric gases, aerosols, and clouds in the atmosphere, climate change may have indirectly affected UV radiation levels in the past.
- These influences are probably going to persist in the future.
<h3>climate:</h3>
- People are at risk from food and water shortages, greater flooding, high heat, an increase in disease, and economic loss due to climate change.
- Conflict and human migration are potential outcomes.
- Climate change is the top hazard to world health in the twenty-first century, according to the World Health Organization (WHO).
<h3>pollutants:</h3>
- these are also resulting in the increase of temperature of the Earth and is also damaging ozone layer.
To learn more about the changes in earth visit:
brainly.com/question/13434833?
#SPJ4
There are different formula you need to keep in mind when solving for [OH-]
Given that pH = 6.10
pH + pOH = 14
6.10 + pOH = 14
pOH = 7.9
[OH-] = 10^(-pOH)
[OH-] = 10^(-7.9)
[OH-] = 0.000000013
[OH-] = 1.3 x 10^-8
<h2>
<u>Answer: [OH-] = 1.3 x 10^-8</u></h2>
