1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Taya2010 [7]
3 years ago
6

When researchers need to prepare many reactions for polymerase chain reaction (PCR) amplification, they often create a "master m

ix" solution. A master mix contains the reagents common to all the planned PCR amplifications, regardless of the target DNA. Making a master mix is a way to minimize the number of pipetting steps.Suppose a researcher needs to PCR amplify seven different genes of interest from different organisms. The researcher prepares a master mix and dispenses it to seven different PCR tubes, one for each gene of interest.Select the PCR components the researcher must add to each of the seven tubes of master mix to selectively amplify each gene of interest.dNTPsMg2+-Mg2+-based bufferprimersDNA polymeraseDNA template
Chemistry
1 answer:
Margaret [11]3 years ago
6 0

Answer:

The master mix contains the following reagents: dNTPs, DNA Polymerase, PCR buffer and MgCl2.

Explanation:

The DNA templates are the gene fragments to amplify by PCR, thereby they have to be added separately in each tube. Moreover, the primer pairs are specific for each gene, thereby they have to be added separately in each tube.  

Deoxynucleotide triphosphates (dNTPs) are the building blocks of the DNA molecules: dGTP, dATP, TTP, and dCTP.

The PCR buffer provides a suitable medium for the activity of the DNA polymerase, often it contains Tris-Hcl and KCl.

MgCl2 is a cofactor for the activity of the DNA Polymerase.

The DNA Polymerase is an enzyme that amplifies DNA by adding nucleotides to the 3' end.

You might be interested in
The _______ are found on the right side of the arrow in a chemical reaction. A. reactants B. products C. subscripts D. coefficie
julia-pushkina [17]

Answer:

B. products are found on the write side of the arrow in a chemical reaction.

8 0
3 years ago
Read 2 more answers
When copper oxide is reacted with sulfuric acid, the acid is often gently heated. This is done in order to:
pav-90 [236]
Copper oxide(solid) + Sulphuric Acid (aqueous)-> Copper Sulphate (aqueous)+ Water(liquid)

In equation form:

CuO +H2SO4 -> CuSO4 + H2O

The colour change you will see is black to blue as Copper oxide is usually found as a black powder. Upon the reaction with sulphuric acid it will change to a cyan blue.

If you heat the made solution of copper sulphate, the water will evaporate and you will be left with white anhydrous copper sulphate crystals.
7 0
3 years ago
A glucose solution that is prepared for a patient should have a concentration of 180 g/L. A nurse has 18 g of glucose. How
Irina-Kira [14]
<h3>Answer:</h3>

0.10 L

<h3>Explanation:</h3>

The concentration of glucose  is given as 180 g/L

The mass of glucose is 18 g

  • Concentration in g/L is calculated by dividing mass of the solute by the volume of the solution.
  • When calculating molarity on the other hand, we divide number of moles of the solute by the volume  of the solution.
  • Concentration in g/L = Mass of solute ÷ Volume

Rearranging the formula,

Volume = Mass of the solute ÷ concentration

             = 18 g ÷ 180 g/L

            = 0.10 L

Therefore, volume of water is 0.10 L

6 0
3 years ago
MgSO4equilibrate the hydrolysis reaction
maksim [4K]

Answer:

just guessing, but hydrolisis indicates ; hydro= h2o. lisis= destruction.

Explanation:

in my training, this wud mean destruction of water, or loss of water in the human body.

2 equalibrate, takes sodium chloride, to retain fluids by IV.

3 0
3 years ago
For an alloy that consists of 91.0 g copper, 111 g zinc, and 7.51 g lead, what are the concentrations (a) of Cu (in at%), (b) of
viktelen [127]

Answer:

\% Cu=45.2\%\\\\\% Zn=53.6\%\\\\\% Pb=1.2\%

Explanation:

Hello!

In this case, given the masses of copper, zinc and lead, it is possible to compute the moles via their atomic masses first:

n_{Cu}=\frac{91.0gCu}{63.55g/mol}=1.432mol\\\\ n_{Zn}=\frac{111gZn}{65.41g/mol}=1.697mol\\\\n_{Pb}=\frac{7.51gPb}{207.2g/mol}=0.0362mol\\

Now, we compute the atomic percentages as shown below:

\% Cu=\frac{1.432}{1.432+1.697+0.0362}*100\% =45.2\%\\\\\% Zn=\frac{1.697}{1.432+1.697+0.0362}*100\%=53.6\%\\\\\% Pb=\frac{0.0362}{1.432+1.697+0.0362}*100\%=1.2\%

Best regards!

4 0
2 years ago
Other questions:
  • Describe a homogeneous mixture
    10·2 answers
  • Magnesium hydroxide is used as an antacid in milk of magnesia and reacts with hydrogen chlrode in the stomach to form water and
    9·1 answer
  • Imagine that you're writing a paper on the topic of ocean acidification. Find two articles about ocean acidification on the Inte
    8·1 answer
  • Aluminum and iron (II) oxide combine to form iron and aluminum oxide.
    9·1 answer
  • ) What mass of potassium nitrate would be needed to
    12·1 answer
  • 6. When does vaporization take place?​
    14·1 answer
  • A mixture of three noble gases has a total pressure of 1.25 atm. The individual pressures exerted by neon and argon are 0.22 atm
    11·1 answer
  • How many grams of O2 are reacted when 3.5<br> mols of P203 are formed?
    12·1 answer
  • How many oxygen atoms are there in one molecule of CH3FO?
    7·1 answer
  • Each iron atom has a mass of about 9 × 10–23 grams. Even a small grain of iron has an incredible number of atoms in it—about 1 x
    14·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!