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Taya2010 [7]
3 years ago
6

When researchers need to prepare many reactions for polymerase chain reaction (PCR) amplification, they often create a "master m

ix" solution. A master mix contains the reagents common to all the planned PCR amplifications, regardless of the target DNA. Making a master mix is a way to minimize the number of pipetting steps.Suppose a researcher needs to PCR amplify seven different genes of interest from different organisms. The researcher prepares a master mix and dispenses it to seven different PCR tubes, one for each gene of interest.Select the PCR components the researcher must add to each of the seven tubes of master mix to selectively amplify each gene of interest.dNTPsMg2+-Mg2+-based bufferprimersDNA polymeraseDNA template
Chemistry
1 answer:
Margaret [11]3 years ago
6 0

Answer:

The master mix contains the following reagents: dNTPs, DNA Polymerase, PCR buffer and MgCl2.

Explanation:

The DNA templates are the gene fragments to amplify by PCR, thereby they have to be added separately in each tube. Moreover, the primer pairs are specific for each gene, thereby they have to be added separately in each tube.  

Deoxynucleotide triphosphates (dNTPs) are the building blocks of the DNA molecules: dGTP, dATP, TTP, and dCTP.

The PCR buffer provides a suitable medium for the activity of the DNA polymerase, often it contains Tris-Hcl and KCl.

MgCl2 is a cofactor for the activity of the DNA Polymerase.

The DNA Polymerase is an enzyme that amplifies DNA by adding nucleotides to the 3' end.

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The central ,innermost or most essential part of something
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3 years ago
How many neutrons does element X have if its atomic number is 31 and its mass number is 80?
MrRissso [65]

49 neutrons in each nucleus.

<h3>Explanation</h3>

For each nucleus:

Mass number = Number of protons + Number of neutrons.

The atomic number of a nucleus is the same as its number of protons. The atomic number of the nucleus here is 31. There are 31 protons in each nucleus.

  • Mass number = 80;
  • Number of protons = Atomic number = 31;
  • The number of neutrons is to be found.

Again,

Mass number = Number of protons + Number of neutrons.

80 = 31 + Number of neutrons.

Number of neutrons = 80 - 31 = 49.

5 0
3 years ago
in this trial, 0.400 M NaOH was added to 40.00 ml of 0.400 M HCl. How many ml of base must be added to cause the colour to chang
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Answer:

The amount of Ml that is needed

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2 years ago
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A. Electrolyte

Explanation:

Concentrated sulfuric acid has a density of 1.84 g/millimeter. When you dilute this with water to 5.20 M, you then have a density of 1.30 g/millimeter, which then can be used as a lead storage for batteries in automobiles. (Got help to answer this at Www.wyzant.com

3 0
3 years ago
Calculate in gramm the mass of 0.1 mole of hydrochloric acid(h=1 ,cl=35.5)​
babunello [35]

Answer:

mass of HCl = 3.65 g

Explanation:

Data Given:

Moles of hydrochloric acid HCl = 0.1 mole

Mass in grams of hydrochloric acid HCl = ?

Solution:

Mole Formula

                  no. of moles = Mass in grams / molar mass

To find Mass in grams rearrange the above Formula

                Mass in grams = no. of moles x molar mass . . . . . . . (1)

Molar mass of HCl = 1 + 35.5 = 36.5 g/mol

Put values in equation 1

              Mass in grams = 0.1 mole x 36.5 g/mol

              Mass in grams = 3.65 g

mass of HCl = 3.65 g

6 0
3 years ago
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