1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Taya2010 [7]
4 years ago
6

When researchers need to prepare many reactions for polymerase chain reaction (PCR) amplification, they often create a "master m

ix" solution. A master mix contains the reagents common to all the planned PCR amplifications, regardless of the target DNA. Making a master mix is a way to minimize the number of pipetting steps.Suppose a researcher needs to PCR amplify seven different genes of interest from different organisms. The researcher prepares a master mix and dispenses it to seven different PCR tubes, one for each gene of interest.Select the PCR components the researcher must add to each of the seven tubes of master mix to selectively amplify each gene of interest.dNTPsMg2+-Mg2+-based bufferprimersDNA polymeraseDNA template
Chemistry
1 answer:
Margaret [11]4 years ago
6 0

Answer:

The master mix contains the following reagents: dNTPs, DNA Polymerase, PCR buffer and MgCl2.

Explanation:

The DNA templates are the gene fragments to amplify by PCR, thereby they have to be added separately in each tube. Moreover, the primer pairs are specific for each gene, thereby they have to be added separately in each tube.  

Deoxynucleotide triphosphates (dNTPs) are the building blocks of the DNA molecules: dGTP, dATP, TTP, and dCTP.

The PCR buffer provides a suitable medium for the activity of the DNA polymerase, often it contains Tris-Hcl and KCl.

MgCl2 is a cofactor for the activity of the DNA Polymerase.

The DNA Polymerase is an enzyme that amplifies DNA by adding nucleotides to the 3' end.

You might be interested in
When a sample of ca(s) loses 1 mole of electrons in a reaction with a sample of o2(g) the oxygen?
Stolb23 [73]
The reaction involved in present case is:

Net Reaction: Ca   +    1/2 O2    →         CaO. ..................(1)

In terms of oxidation and reduction, the reaction can be shown at

Oxidation: Ca     →       Ca2+      +       2e- .................(2)
Reduction: 1/2O2    +   2e-      →      O2-...................(3)

From, reaction 1 it can be seen that 1 mol of Ca reacts with 1/2 mol of O2 to form 1 mol of CaO.

From, reaction 2 it can be seen that 1 mol of Ca, generates 2 mol of e-.

Thus, when 1/2 mol of Ca is used in reaction, it will lose 1 mol of electrons.
6 0
4 years ago
Read 2 more answers
One way to identify the type of radioactive decay produced in a reaction is to pass the emission through an electric field Descr
Elena L [17]

Decay of Uranium-238 produces gamma radiaton whereas decay of thorium-234 releases beta radiation.

<h3>What type of radiation is produced?</h3>

In the decay of U-238, two gamma rays of different energies are emitted in addition to the alpha particle while on the other hand, in the decay of thorium-234 , beta rays are emitted.

So we can conclude that decay of Uranium-238 produces gamma radiaton whereas decay of thorium-234 releases beta radiation.

Learn more about decay here: brainly.com/question/25537936

5 0
3 years ago
Put the following elements into five pairs of elements that have similar chemical reactivity: F, Sr, P, Ca, O, Br, Rb, Sb, Li, S
kirill115 [55]

Explanation:

Given elements:

  F, Sr, P, Ca, O, Br, Rb, Sb, Li, S

Elements with the same chemical reactivity will belong to the same group on the periodic table. This implies that elements in the same column will have the same reactivity;

  Li and Rb are both alkali metals in group 1

  Ca and Sr are both alkali earth metals in group 2

  F and Br are halogens in group 7

  O and S are group 6 elements

 P and Sb are both in group 5 on the periodic table

So these groupings show elements with the same chemical properties.

7 0
3 years ago
Which statement best describes the organelles in a cell
Bas_tet [7]

Answer:

(2) Organelles must work together and their

activities must be coordinated

Explanation:

Organelles are usually located in cells. They are saddled with the role of performing specific functions in the cells for the overall functioning of life. In eukaryotic cells, the organelles are membrane bounded but in prokaryotic or primitive cells such is not the case.

Examples of cell organelles are ribosome, food vacuole, nucleus e.t.c. Just like organs in the body, organelles must work together in order to enhance life.

5 0
3 years ago
One way in which elements differ from each other is the structure of the electron cloud in each elements Atoms in electron cloud
BartSMP [9]

Not sure what you are asking. I have two possible answers though...

It could either be more negatively charged, or valence electrons.

The more away from the nucleus a electron is, the more negatively charged it is.

The electrons on the outermost electron shell is valence electrons.

Again, I don't know what you were asking, but one of these answers may be correct.

4 0
3 years ago
Read 2 more answers
Other questions:
  • What is the molarity of sodium chloride in solution that is 13.0% by mass sodium chloride and that has a density of 1.10 g/mL?
    12·1 answer
  • A container holds 500. ML of CO2 at 20.° C and 742 torr. What will be the volume of the CO2 if the pressure is increased to 795
    5·2 answers
  • MgCl2 + NH4NO3 --&gt; Mg NO3 + NH4Cl is this a double replacement reaction?
    14·1 answer
  • At what temperature does uranium hexafluoride have a density of 0.5820 g/L at 0.5073 atm?
    6·1 answer
  • Write the chemical formulas for the ionic compound vanadium (v) oxide
    14·1 answer
  • Which statement is true with respect to the investigation?
    12·1 answer
  • What are the amplitude's for both waves A and B?
    13·1 answer
  • Which of the following statements correctly describes the characteristics of the surface features in Column A and Column B?
    14·1 answer
  • A certain liquid has a normal freezing point of and a freezing point depression constant . Calculate the freezing point of a sol
    14·1 answer
  • A
    6·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!