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Taya2010 [7]
3 years ago
6

When researchers need to prepare many reactions for polymerase chain reaction (PCR) amplification, they often create a "master m

ix" solution. A master mix contains the reagents common to all the planned PCR amplifications, regardless of the target DNA. Making a master mix is a way to minimize the number of pipetting steps.Suppose a researcher needs to PCR amplify seven different genes of interest from different organisms. The researcher prepares a master mix and dispenses it to seven different PCR tubes, one for each gene of interest.Select the PCR components the researcher must add to each of the seven tubes of master mix to selectively amplify each gene of interest.dNTPsMg2+-Mg2+-based bufferprimersDNA polymeraseDNA template
Chemistry
1 answer:
Margaret [11]3 years ago
6 0

Answer:

The master mix contains the following reagents: dNTPs, DNA Polymerase, PCR buffer and MgCl2.

Explanation:

The DNA templates are the gene fragments to amplify by PCR, thereby they have to be added separately in each tube. Moreover, the primer pairs are specific for each gene, thereby they have to be added separately in each tube.  

Deoxynucleotide triphosphates (dNTPs) are the building blocks of the DNA molecules: dGTP, dATP, TTP, and dCTP.

The PCR buffer provides a suitable medium for the activity of the DNA polymerase, often it contains Tris-Hcl and KCl.

MgCl2 is a cofactor for the activity of the DNA Polymerase.

The DNA Polymerase is an enzyme that amplifies DNA by adding nucleotides to the 3' end.

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Answer:

Hope this helped :) good luck! ❤️

Explanation:

A <em>coolant solution</em> is a <u><em>homogeneous </em></u>mixture because the coolant particles are not chemically combined with the water (keep their properties) and they are evenly distributed throughout the water.

7 0
3 years ago
Match these items. 1. moles per liter of solution solute 2. spreading out Keq 3. substance being dissolved diffusion 4. general
Degger [83]

Answer:

1. moles per liter of solution - Molarity

2. spreading out - diffusion

3. substance being dissolved- solute

4. general equilibrium constant - Keq  

5. the substance doing the dissolving in a solution -  solvent

6. solubility product constant - Ksp

Explanation:

Number of moles of substance being dissolved in one liter of solution  is defined as molarity  

Solute is the substance which is dissolved and solvent is the substance into which the solute is dissolved. For example salt is solute and water is solvent.

Ksp defines the solubility product constant which indicates the equilibrium between a solid and solution and the number of ions of solid required to achieve this equilibrium.

Keq depicts the scenario where reactant and product concentrations are constant in a chemical reaction.  

Hence, the correct match is

1. moles per liter of solution - Molarity

2. spreading out - diffusion

3. substance being dissolved- solute

4. general equilibrium constant - Keq  

5. the substance doing the dissolving in a solution -  solvent

6. solubility product constant - Ksp

8 0
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As a human being, we are considered to be a ..... because we rely on other organism for food​
ryzh [129]
Heterotrophs i think :)

3 0
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Answer:

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3 years ago
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