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Taya2010 [7]
3 years ago
6

When researchers need to prepare many reactions for polymerase chain reaction (PCR) amplification, they often create a "master m

ix" solution. A master mix contains the reagents common to all the planned PCR amplifications, regardless of the target DNA. Making a master mix is a way to minimize the number of pipetting steps.Suppose a researcher needs to PCR amplify seven different genes of interest from different organisms. The researcher prepares a master mix and dispenses it to seven different PCR tubes, one for each gene of interest.Select the PCR components the researcher must add to each of the seven tubes of master mix to selectively amplify each gene of interest.dNTPsMg2+-Mg2+-based bufferprimersDNA polymeraseDNA template
Chemistry
1 answer:
Margaret [11]3 years ago
6 0

Answer:

The master mix contains the following reagents: dNTPs, DNA Polymerase, PCR buffer and MgCl2.

Explanation:

The DNA templates are the gene fragments to amplify by PCR, thereby they have to be added separately in each tube. Moreover, the primer pairs are specific for each gene, thereby they have to be added separately in each tube.  

Deoxynucleotide triphosphates (dNTPs) are the building blocks of the DNA molecules: dGTP, dATP, TTP, and dCTP.

The PCR buffer provides a suitable medium for the activity of the DNA polymerase, often it contains Tris-Hcl and KCl.

MgCl2 is a cofactor for the activity of the DNA Polymerase.

The DNA Polymerase is an enzyme that amplifies DNA by adding nucleotides to the 3' end.

You might be interested in
A solution is made by dissolving 4.87 g of potassium nitrate in water to a final volume of 86.4 mL solution. What is the weight/
lara31 [8.8K]

Answer:

A solution is made by dissolving 4.87 g of potassium nitrate in water to a final volume of 86.4 mL solution. The weight/weight % or percent by mass of the solute is :

<u>2.67%</u>

Explanation:

Note : Look at the density of potassium nitrate in water if given in the question.

<u><em>You are calculating </em></u><u><em>weight /Volume</em></u><u><em> not weight/weight % or percent by mass of the solute</em></u>

Here the <u>weight/weight % or percent by mass</u> of the solute is asked : So first convert the<u> VOLUME OF SOLUTION into MASS</u>

Density of potassium nitrate in water KNO3 = 2.11 g/mL

density=\frac{mass}{volume}

Density = 2.11 g/mL

Volume of solution = 86.4 mL

2.11=\frac{mass}{86.4}

mass = 2.11\times 86.4

mass=182.3grams

Mass of Solute = 4.87 g

Mass of Solution = 183.2 g

w/w% of the solute =

= \frac{mass\ of\ solute}{mass\ of\ solution}\times 100

=\frac{4.87}{183.2}\times 100

w/w%=2.67%

8 0
3 years ago
Which determines how atoms combine to form molecules?
arlik [135]
Will be letter E cause all of them can combine molecule
7 0
4 years ago
How many sigma and pi bonds does it contain ch3chchco2h?
ira [324]
Answer:
            But-2-enoic acid has 11 Sigma Bonds and 2 Pi Bonds.

Explanation:
                   The sigma bonds which are formed due to head to head overlap of partally filled orbitals are shown in red color, while Pi bonds which are formed after the formation of sigma bond by overlap of orbitals perpendicular to the sigma bond are shown in blue color.

8 0
3 years ago
Which concept does the diagram show?
salantis [7]

Answer:

temperature

Explanation:

sorry if wrong

4 0
3 years ago
PLEASE HELP NEED THIS TO PASS!!!
nadya68 [22]

(1)

At phase A, the water is fully solid, At phase C the water is fully liquid and at phase E the water is fully gas. At this ponts all the substance is in one state other than the fact that its molecules are getting more excited as they gain more energy. This is why there is an increase in temperatures. At the transition points water is changing phase and therefore at any point in between the transition not all water will be in the same phase.

(2)

Point F

This is because the solid water stops rising in temperatures and begins changing phase into liquid water. This is the highest temperatures that the solid water can attain without turning into liquid. You can see from the graph that the temperatures rise at this point levels off between point F and G  

(3)

Point H

This is because the liquid water stops rising in temperatures and begins changing phase into gaseous form. This is the highest temperatures that the liquid water can attain without turning into gas/water vapor. You can see from the graph that the temperatures rise  at this point levels off between point H and I.

(4)

The energy is increasing

You can see from the graph that energy is being absorbed by the water (due to increases in energy on the x-axis on the graph) but there is no corresponding rise in temperatures in y-axis. This means the energy begin absorbed is being used to change the phase of the water.

(5)

The water is changing phases

Because the energy absorbed is being used to change the phase of the liquid. The energy is being used to break the bonds between the molecules so the molecules become farther apart and causing a change in phase on the water. Therefore the vibrational moments of the molecules (responsible for rising in temperatures) remain the same over this latent phase. This energy absorbed to change phase is called latent energy/heat.  

Learn More:

For more on change of phase of water check out;

brainly.com/question/2128466

brainly.com/question/13251064

#LearnWithBrainly

3 0
3 years ago
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