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Taya2010 [7]
3 years ago
6

When researchers need to prepare many reactions for polymerase chain reaction (PCR) amplification, they often create a "master m

ix" solution. A master mix contains the reagents common to all the planned PCR amplifications, regardless of the target DNA. Making a master mix is a way to minimize the number of pipetting steps.Suppose a researcher needs to PCR amplify seven different genes of interest from different organisms. The researcher prepares a master mix and dispenses it to seven different PCR tubes, one for each gene of interest.Select the PCR components the researcher must add to each of the seven tubes of master mix to selectively amplify each gene of interest.dNTPsMg2+-Mg2+-based bufferprimersDNA polymeraseDNA template
Chemistry
1 answer:
Margaret [11]3 years ago
6 0

Answer:

The master mix contains the following reagents: dNTPs, DNA Polymerase, PCR buffer and MgCl2.

Explanation:

The DNA templates are the gene fragments to amplify by PCR, thereby they have to be added separately in each tube. Moreover, the primer pairs are specific for each gene, thereby they have to be added separately in each tube.  

Deoxynucleotide triphosphates (dNTPs) are the building blocks of the DNA molecules: dGTP, dATP, TTP, and dCTP.

The PCR buffer provides a suitable medium for the activity of the DNA polymerase, often it contains Tris-Hcl and KCl.

MgCl2 is a cofactor for the activity of the DNA Polymerase.

The DNA Polymerase is an enzyme that amplifies DNA by adding nucleotides to the 3' end.

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If it requires 18.2 milliliters of 0.45 molar barium hydroxide to neutralize 38.5 milliliters of nitric acid, solve for the mola
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4 years ago
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A sample of a compound containing boron (B) and hydrogen (H) contains 5.443 g of B and 1.522 g of H. The molar mass of the compo
Svetach [21]

You are calculating the empirical formula of this chemical compound, which is the question with moles, molar mass, and number of moles.

first you divide the mass of BORON by its molar mass(relative formula mass)because there is a formula about moles state: number of moles=mass/molar mass.

So, 5.443/11 is about 0.5. Then, the RFM of H is 1, so the number of mole is 1.522/1=1.522.

Next, you get the number of moles in order is; 0.5 and 1.522. Now we need to look at the ratio between these numbers. 0.5 is smaller so we use it as the ratio of 1.  next use 1.522/0.5 is 3.044 which has a greatest common factor of 3. so the empirical formula is BH3.

Now we are going to solve the molecular formula.

the molar mass ofthis compound is 30g, so we're going to find the RFM of the empirical formulsof BH3 first.

11+3=14.

now we see how many times 14 goes into 30. 30/14=2.14 which is about 2.

So now we need to times the subscript of the empirical formula by two.

thus, the molecular formula is B2H6.


To solve this kind of  questions, there are many steps:Know what you are calculating about, it's about the molecular formula, so you need to find out the number of moles of each elements. then use the molar mass of the whole compound to calculate the molecular formula.

1) Find the RFM of the element, because that is the molar mass(mass of 1 mole) of this element.

2) number of moles= mass/molar mass. use this formula to help you get the number of moles of each element in this compound

3) look at the relationship between the number of moles of each elements. find out the ratio between them.

4) then use the molarmass of the whole compound to find the molecular formula. molar mass of the whole compound/RFM(molar mass) of the empirical formula of elements= the number you need to multiply by the subscript of the empirical formula to get the molecular formula.

please tell me if i got anything wrong;)



4 0
3 years ago
An insoluble base reacts with an acid to form a salt
Alex17521 [72]

Answer: you can use a strainer

Explanation:

4 0
3 years ago
Calculate the equilibrium constant of the reaction below if the pressures are 1.0atm, 2.0 atm, and 1.0 atm respectively. PCl3 +
Makovka662 [10]

Answer:

K = 0.5

Explanation:

Based on the reaction:

PCl₃ + Cl₂ ⇄ PCl₅

The equilibrium constant, K, is defined as:

K = P PCl₅ / P PCl₃ * P Cl₂

<em>Where P represent the pressure at the equilibrium for each one of the gases involved in the equilibrium.</em>

<em />

As:

P PCl₅ = 1.0atm

P PCl₃ = 1.0atm

P Cl₂ = 2.0atm

K = 1.0atm / 1.0atm * 2.0atm

<h3>K = 0.5</h3>
7 0
2 years ago
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