1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
QveST [7]
3 years ago
10

Biochemistry The famous Anfinsen protein folding experiment was the first to show that the amino acid sequence of a protein can

be sufficient to determine its structure. This experiment used the enzyme ribonuclease A, which contains four disulfide bonds. First, urea and beta-mercaptoethanol were added to a solution of RNase A, with the result that protein activity was lost. Next, urea was slowly removed by dialysis. Finally, beeta-mercaptoethanol was removed by dialysis, with the result that almost all of the original protein activity was regained. What happens when the order of the last two steps is swapped: beta-mercaptoethanol is removed first, then urea is removed.
A) Non-native disulfide bonds form after beta-mercaptoethanol is removed, so the protein cannot refold correctly
B) Half the original activity is regained
C) The original disulfide bonds reform, but the protein still cannot refold
D) The disulfide bonds do not reform, but activity is regained anyway
E) The protein still refolds the same way.
Chemistry
1 answer:
OverLord2011 [107]3 years ago
5 0

Answer:

The A option is the correct answer: Non-native disulfide bonds form after beta-mercaptoethanol is removed, so the protein cannot refold correctly

Explanation:

Beta-Mercaptoehanol is responsible to reduce the four disulfide bonds present in ARNase; Urea deals with non covalente bonds.  In presence of both ARNase is denatured.

If Urea is first removed by dialysis, and later is removed Beta-Mercaptoethanol, the enzyme recovers ist activity.

If Beta-Mercaptoethanol is first removed, disulfide bonds different from native use to be formed.  As a result ARNase is not an active enzyme

You might be interested in
If the temperature of a gas is increased from 20°C to 35°C, what is the new pressure if the original pressure was 1.2 atm? Assum
Marta_Voda [28]

Answer:

B.) 1.3 atm

Explanation:

To find the new pressure, you need to use Gay-Lussac's Law:

P₁ / T₁ = P₂ / T₂

In this equation, "P₁" and "T₁" represent the initial pressure and temperature. "P₂" and "T₂" represent the final pressure and temperature. After converting the temperatures from Celsius to Kelvin, you can plug the given values into the equation and simplify to find P₂.

P₁ = 1.2 atm                                    P₂ = ? atm

T₁ = 20 °C + 273 = 293 K              T₂ = 35 °C + 273 = 308 K

P₁ / T₁ = P₂ / T₂                                             <----- Gay-Lussac's Law

(1.2 atm) / (293 K) = P₂ / (308 K)                  <----- Insert values

0.0041 = P₂ / (308 K)                                   <----- Simplify left side

1.3 = P₂                                                         <----- Multiply both sides by 308

3 0
1 year ago
What is the oxidation number for each atom in NH4CI?
Alika [10]
<span>N = +3, H = +1 ,Cl = -1
</span><span>

</span>
5 0
3 years ago
A person's heart beats 72 times a minute. At this rate, how many times does it beat in one hour?
sesenic [268]

At one minute, a persons's heart beats 72 times.

Therefore, in one hour or 60 minutes, a person's heart will beat (60×72) times, i.e., 4320 times

Answer is 4320 times

6 0
2 years ago
Convert 2.345 centimeters cubed to inches cubed. 1inch = 2.54cm
GalinKa [24]

Answer:

0.92322835

Explanation:

0.92322835 rounded= 0.9 or 0.92

3 0
3 years ago
Read 2 more answers
Can someone pls help, I’m sorry if it’s a lot
Helga [31]
Answer: 2738


Explaining: you gotta add
3 0
3 years ago
Other questions:
  • Neon is an inert gas and has the atomic number of 10. Which of the following correctly depicts its electron configuration? 1s22d
    12·1 answer
  • Which formula is a molecular compound?<br><br> H2O2<br> CaO<br> MgS<br> NaCl
    10·2 answers
  • What is the temperature when ice Melt
    8·1 answer
  • In an ionic bond:
    15·2 answers
  • How to learn chemistry?​
    12·1 answer
  • Spiral galaxies have a central core from which curved arms spiral outward. True False
    9·2 answers
  • The first excited electronic energy level of the helium atom is 3.13 x 10-18 J above the ground level. Estimate the temperature
    6·1 answer
  • PART A: Which of the following identifies the central idea of the text? The text is the power of advertising
    5·2 answers
  • H
    7·1 answer
  • renata developed a hair dye additive that is guaranteed to cover grey hair. she granted the right to use this additive to a majo
    15·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!