Hey there!
Values Ka1 and Ka2 :
Ka1 => 8.0*10⁻⁵
Ka2 => 1.6*10⁻¹²
H2A + H2O -------> H3O⁺ + HA⁻
Ka2 is very less so I am not considering that dissociation.
Now Ka = 8.0*10⁻⁵ = [H3O⁺] [HA⁻] / [H2A]
lets concentration of H3O⁺ = X then above equation will be
8.0*10−5 = [x] [x] / [0.28 -x
8.0*10−5 = x² / [0.28 -x ]
x² + 8.0*10⁻⁵x - 2.24 * 10⁻⁵
solve the quardratic equation
X =0.004693 M
pH = -log[H⁺]
pH = - log [ 0.004693 ]
pH = 2.3285
Hope that helps!
Answer:
Taq polymerase is a thermostable which states that it can even work at higher temperature. The main function of using this enzyme is that it is used to amplify the DNA which will help in producing ample amount of DNA sample.
The enzyme activity is temperature dependent. The denaturation and annealing steps of the PCR occurs at very high temperature.
Any other enzyme used at such an high temperature would have decreased the enzyme activity and the procedure would not be completed.
$724.73 this would be the answer because if you subtract 320.50 and 86.10 from the 1056.33 then add 75 you get 724.73
277.79 atm is the calculated gas pressure.
The ideal gas is a fictitious concept used to study how real gases behave by comparing them to their deviations. The pressure-temperature rules are followed by an ideal gas.
177 atm is the initial pressure. The starting temperature is 298 K (25 °C = 25 + 273 °C).
195°C = 195+273
= 468K is the final temperature.
The pressure temperature relation illustrated below can be used to get the final pressure.
P1/T1 = P2/T1
= P1T2/T1
= 177 atm 468 K /298 K
= 277.97 atm
The final pressure is therefore 277.97 atm.
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