Ion exchange chromatography is a chemical process where molecules are separated by affinity to an ion exchange resin. To separate different aminoacids you must use the isoelectric point (That is the pH where the aminoacid will be in its neutral form).

For lysine, PI is:

$pH = \frac{1}{2} (9,1+10,5) =$ 9,8

For arginine:

$pH = \frac{1}{2} (9,0+12,5) =$ 10,75

At pH = 9,8 lysine will be in its neutral form and will not be retain in the column but arginine will be in +1 charge being retained by the ion exchange resin.

Thus, <em>pH 9,8 is likely to work best for this separation</em>

I hope it helps!

5 0

3 years ago

I need help with number two too?

KIM [24]

1. 20s, 22s, and 30s... height is the highest.

2. 34s, 38s, and 41s..... height is at its lowest.

i could be wrong.... but i tried.

8 0

3 years ago

Natural remedies are usually more effective and safer because they have thoroughly tested .

mihalych1998 [28]

The answer for this question would be B) False or the second option.

Answer is FALSE: ✅

6 0

3 years ago

Making models involves creating representations of complex objects or processes to help people study and understand things that

swat32

Somewhat false
observations can be made of a model of the statue of liberty, say, or in real line

6 0

2 years ago