What factors are requierd for cloning

1 answer:

ioda3 years ago

8 0

In mammals and amphibians? An enucleated egg, a donor nucleus (preferably from an early developmental stage such as a blastocyst), and a means to stimulate the egg to be activated as if it had just been fertilized (poking with a needle is sometimes enough)

Or cloning into a vector as in at the level of DNA?
A vector with positve and negative selection markers (like antibiotic resistance and drug susceptibility), an insert, DNA ligase and restriction enzymes, buffer for restriction and ligation. Or if you are doing cloning by PCR, then you need primers, template, nucleotides, RNA pol like Taq polymerase etc.

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Which of these elements have the same number of valance electrons

solmaris [256]

Both carbon and lead belong to Group IV elements, and thus they have the same number of valence electrons.
In each of the other options, the two elements belong to different groups, and thus they do NOT have the same number of valence electrons.

I hope this helped you, please tell me if I am correct or not <3

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4 years ago

Please refer to image please

GarryVolchara [31]

Answer:

76.9L

Explanation:

Based on the graph, whenever the temperature increases by 100K, the volume increases by 10L, so do 769/10

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2 years ago

How many grams will be needed

klio [65]

787.57 grams GIVE ME BRAINLIEST

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3 years ago

Please help me with this.

zlopas [31]

Answer A: Connect a wire coil to an ammeter. Move a bar magnet into and out of the wire coil as you observe the ammeter.

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3 years ago

I NEED HELP PLEASE! :)

MrMuchimi

answer 1 :

Law of conservation of momentum states that

For two or more bodies in an isolated system acting upon each other, their total momentum remains constant unless an external force is applied. Therefore, momentum can neither be created nor destroyed.

answer 2: 

When a substance is provided energy in the form of heat, it's temperature increases. The extent of temperature increase is determined by the heat capacity of the substance. The larger the heat capacity of a substance, the more energy is required to raise its temperature.

When a substance undergoes a FIRST ORDER phase change, its temperature remains constant as long as the phase change remains incomplete. When ice at -10 degrees C is heated, its temperature rises until it reaches 0 degrees C. At that temperature, it starts melting and solid water is converted to liquid water. During this time, all the heat energy provided to the system is USED UP in the process of converting solid to the liquid. Only when all the solid is converted, is the heat used to raise the temperature of the liquid.

This is what results in the flat part of the freezing/melting of condensation/boiling curve. In this flat region, the heat capacity of the substance is infinite. This is the famous "divergence" of the heat capacity during a first order phase transition.

There are certain phase transitions where the heat capacity does not become infinitely large, such as the process of a non-magnetic substance becoming a magnetic substance (when cooled below the so-called Curie temperature).

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3 years ago