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3 years ago

What factors are requierd for cloning

1 answer:

ioda3 years ago

8 0

In mammals and amphibians? An enucleated egg, a donor nucleus (preferably from an early developmental stage such as a blastocyst), and a means to stimulate the egg to be activated as if it had just been fertilized (poking with a needle is sometimes enough)

Or cloning into a vector as in at the level of DNA?
A vector with positve and negative selection markers (like antibiotic resistance and drug susceptibility), an insert, DNA ligase and restriction enzymes, buffer for restriction and ligation. Or if you are doing cloning by PCR, then you need primers, template, nucleotides, RNA pol like Taq polymerase etc.

You might be interested in

How many grams of Sg is required to produce 83.10 g SF6? S: +24F-->8SF

ozzi

Answer : The mass of $S_8$ required is 18.238 grams.

Explanation : Given,

Mass of $SF_6$ = 83.10 g

Molar mass of $SF_6$ = 146 g/mole

Molar mass of $S_8$ = 256.52 g/mole

The balanced chemical reaction is,

$S_8+24F_2\rightarrow 8SF_6$

First we have to determine the moles of $SF_6$ .

$\text{Moles of }SF_6=\frac{\text{Mass of }SF_6}{\text{Molar mass of }SF_6}=\frac{83.10g}{146g/mole}=0.569moles$

Now we have to determine the moles of $S_8$ .

From the balanced chemical reaction we conclude that,

As, 8 moles of $SF_6$ produced from 1 mole of $S_8$

So, 0.569 moles of $SF_6$ produced from $\frac{0.569}{8}=0.0711$ mole of $S_8$

Now we have to determine the mass of $S_8$ .

$\text{Mass of }S_8=\text{Moles of }S_8\times \text{Molar mass of }S_8$

$\text{Mass of }S_8=(0.0711mole)\times (256.52g/mole)=18.238g$

Therefore, the mass of $S_8$ required is 18.238 grams.

7 0

3 years ago

What is the viscosity and flammability of refinery gas?

Liono4ka [1.6K]

Hydrocarbons

Hope this helps :)

6 0

2 years ago

What happens if the metal you throw in is MORE REACTIVE than the<br> metal ion in solution?

IRISSAK [1]

A displacement reaction Would occur in this situation

6 0

3 years ago

What is wrong with this model of the atom.

sp2606 [1]

Answer:

A. The neutrons and electrons are in the wrong place.

Explanation:

The atom's nucleus contains both protons and neutrons, whilst the electrons are arranged in shells around the nucleus.

8 0

2 years ago

A 25.0-mL sample of 0.150 M hydrazoic acid, HN3, is titrated with a 0.150 M NaOH solution. What is the pH after 13.3 mL of base

tamaranim1 [39]

Answer:

pH ≅ 4.80

Explanation:

Given that:

the volume of HN₃ = 25 mL = 0.025 L

Molarity of HN₃ = 0.150 M

number of moles of HN₃ = 0.025 × 0.150

number of moles of HN₃ = 0.00375 mol

Molarity of NaOH = 0.150 M

the volume of NaOH = 13.3 mL = 0.0133

number of moles of NaOH = 0.0133× 0.150

number of moles of NaOH = 0.001995 mol

The chemical equation for the reaction of this process can be written as:

$HN_3 + OH- ---> N^-_{3} + H_2O$

1 mole of hydrazoic acid react with 1 mole of hydroxide to give nitride ion and water

thus the new number of moles of HN₃ = 0.00375 - 0.001995 = 0.001755 mol

Total volume used in the reaction = 0.025 + 0.0133 = 0.0383 L

Concentration of $HN_3$ = $\dfrac{0.001755}{0.0383}$ = 0.0458 M

Concentration of $N^{-}_3$ = $\dfrac{ 0.001995 }{0.0383}$ = 0.0521 M

GIven that :

Ka = $1.9 x 10^{-5}$

Thus; it's pKa = 4.72

$pH =4.72 + log(\dfrac{ \ 0.0521}{0.0458})$

$pH =4.72 + log(1.1376)$

$pH =4.72 + 0.05598$

$pH =4.77598$

pH ≅ 4.80

3 0

2 years ago