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sladkih [1.3K]
3 years ago
15

What factors are requierd for cloning

Chemistry
1 answer:
ioda3 years ago
8 0
In mammals and amphibians? An enucleated egg, a donor nucleus (preferably from an early developmental stage such as a blastocyst), and a means to stimulate the egg to be activated as if it had just been fertilized (poking with a needle is sometimes enough) 

Or cloning into a vector as in at the level of DNA? 
A vector with positve and negative selection markers (like antibiotic resistance and drug susceptibility), an insert, DNA ligase and restriction enzymes, buffer for restriction and ligation. Or if you are doing cloning by PCR, then you need primers, template, nucleotides, RNA pol like Taq polymerase etc.
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