1 1. 23.5 meters to kilometers.
1 answer:
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a.) Asp and Lys
Asp will elute first from the column because it has less positively charged functional groups than Lys.
b.) Arg and Met
Met will elute first from the column because it has less positively charged functional groups than Lys.
c.) Glu and Val
Glu will elute first from the column because it has more negativity functional groups than Lys and will be not be much retained by the -SO₃⁻ groups from the ion-exchange coloumn.
d.) Gly and Val
Gly will elute first from the column because Lys have a longer alkyl chain which will be attracted by the strongly hydrophobic backbone for the resin.
e.) Ser and Ala
Ser will be eluted first from the column because Ala alkyl chain will be more attracted by the strongly hydrophobic backbone for the resin. Ser have an -OH group which will decrease the hydrophobicity of the alkyl chain and will not be so much retained on the column.
<u>Answer:</u> The molecular weight of protein is
<u>Explanation:</u>
To calculate the concentration of solute, we use the equation for osmotic pressure, which is:
or,
where,
= Osmotic pressure of the solution = 0.0861 atm
i = Van't hoff factor = 1 (for non-electrolytes)
= mass of protein = 400 mg = 0.4 g (Conversion factor: 1 g = 1000 mg)
= molar mass of protein = ?
= Volume of solution = 5.00 mL
R = Gas constant =
T = temperature of the solution =
Putting values in above equation, we get:
Hence, the molecular weight of protein is