Answer:
Adding a reducing agent
Explanation:
Homodimeric proteins are formed by non-covalent interactions between two identical proteins (monomers).
Some of these intermolecular interactions are mediated by disulphide bonds which can be broken by a reducing agent like beta-mercaptoethanol (2-ME).
By adding the reducing agent to the gel, it is probable that the dimer is broken and the band observed will correspond to the two identical 22.5-kDa monomers.
Given Information:
Radius = r = 0.5 m
Magnetic field = 1.0 T
Required Information:
Period = T = ?
Speed = v = ?
Kinetic energy = KE = ?
Answer:
Period = 0.13x10⁻⁶ seconds
speed = 24.16x10⁶ m/s
Kinetic energy = 12.11 MeV
Explanation:
(a) period
The time period of alpha particle is related to its orbital speed as
T = 2πr/v eq. 1
According to newton's law
F = ma
Force due to magnetic field is given by
F = qvB
qvB = ma
qvB = m(v²/r)
qB = mv/r
v = qBr/m eq. 2
substitute the eq. 2 in eq. 1
T = 2πr/qBr/m
r cancels out
T = 2π/qB/m
T = 2πm/qB
T = 2π*6.65x10⁻²⁷/2*1.602x10⁻¹⁹*1
T = 0.13x10⁻⁶ seconds
(b) speed
From equation 1
T = 2πr/v
v = 2πr/T
v = 2π*0.5/0.13x10⁻⁶
v = 24.16x10⁶ m/s
(c) kinetic energy (in electron volts)
Kinetic energy is given by
KE = 0.5mv²
KE = 0.5*6.65x10⁻²⁷*(24.16x10⁶)²
KE = 1.94x10⁻¹² J
since 1 electron volt has 1.602x10⁻¹⁹ J
KE = 1.94x10⁻¹²/1.602x10⁻¹⁹
KE = 12.11 MeV
All of that fluff at the beginning is interesting, but completely irrelevant
to the question. The question is just asking for the mass of an object
that weighs 3.6N on Earth.
Weight = (mass) x (acceleration of gravity)
3.6N = (mass) x (9.8 m/s²)
Divide each side
by 9.8 m/s : Mass = 3.6N / 9.8 m/s² = <em>0.367 kilogram</em> (rounded)
Compared with an Earth year, a galactic year represents time on a grand scale but its not a consistent measurement across the galaxy