On your first day working in a laboratory, you are told to make up 1 Liter of a 0.5 Molar Tris solution with a pH of 7.4. You me
asure out the appropriate amount of Tris base and dissolve it in water. You then measure the pH and find out that it has an initial pH of 11. You add some concentrated HCl, and the pH begins to drop. However, as you approach the target pH, you find that you need to add large amounts of HCl to lower the pH even a small amount. If the molecular weight of Tris base is 121.14, how many grams did you use
The concentration of the Tris solution buffer is 0.5 molar (That is, 0.5 moles per liter). As you need to make 1L, you need to have in the solution <em>0.5 moles of TRIS.</em>
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To convert these moles of TRIS to grams you need to use the molecular weight of TRIS base (121.14g/mol), thus:
