What is the process of removing the bacteria?
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Biphenyl will have a higher R value than the Methyl Orange.
Explanation:
Biphenyl is a aromatic hydrocarbon and it is a nonpolar molecule.
Methyl Orange is a organic compound with a -SO₃⁻Na⁺ polar functional group which will induce a high polarity in the compound.
You may find the chemical structures of both molecules in the attached picture.
Column chromatography, which use as stationary phase silica gel, is a good technique for separation of the Methyl Orange from Biphenyl.
Being a non-polar molecule, Biphenyl will have a higher R value than the Methyl Orange.
To separate them you use a appropriate solvent as eluent, as exemple chloroform, and Biphenyl will elute first from the column and after that, as a separate phase, Methyl Orange will elute thus separating them.
Learn more about:
chromatography
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