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xxMikexx [17]
2 years ago
12

What is the temperature used for the extension step?

Physics
1 answer:
lisabon 2012 [21]2 years ago
8 0
<h2>72°C</h2>

Explanation:

  • The polymerase chain response (PCR) could be a strategy to quickly open up groupings of DNA.
  • Amid a normal PCR, format DNA (containing the locale of intrigued) is blended with deoxynucleotides (dNTPs), a DNA polymerase and small segments of DNA called primers.
  • PCR involves a series of temperature cycles.
  • The temperature used for the extension step is 72°C.
  • The temperature that is used during the extension phase is dependent on the DNA polymerase that is used.
  • Amid the expansion step (ordinarily 68°C-72°C) the polymerase amplifies the groundwork to create an early DNA strand. This prepare is rehashed different times
  • Generally at this stage, the reaction mixture is heated to a temperature intermediate between the denaturation and annealing temperatures. The optimal temperature for Taq polymerase is 72°C.
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