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xxMikexx [17]
3 years ago
12

What is the temperature used for the extension step?

Physics
1 answer:
lisabon 2012 [21]3 years ago
8 0
<h2>72°C</h2>

Explanation:

  • The polymerase chain response (PCR) could be a strategy to quickly open up groupings of DNA.
  • Amid a normal PCR, format DNA (containing the locale of intrigued) is blended with deoxynucleotides (dNTPs), a DNA polymerase and small segments of DNA called primers.
  • PCR involves a series of temperature cycles.
  • The temperature used for the extension step is 72°C.
  • The temperature that is used during the extension phase is dependent on the DNA polymerase that is used.
  • Amid the expansion step (ordinarily 68°C-72°C) the polymerase amplifies the groundwork to create an early DNA strand. This prepare is rehashed different times
  • Generally at this stage, the reaction mixture is heated to a temperature intermediate between the denaturation and annealing temperatures. The optimal temperature for Taq polymerase is 72°C.
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A proton is released in a uniform electric field, and it experiences an electric force of 2.04×10−14 N toward the south.
Lemur [1.5K]

Answer:

Part A:

E=127500N/C\\E=1.275*10^{5} N/C

Part B:

Option B (Towards the South)

Explanation:

Part A:

Magnitude if electric field E:

E=Force/charge

Force=2.04×10−14 N

Charge=1.6×10−19 C

E=\frac{2.04*10^{-14}}{1.6*10^{-19}} \\E=127500N/C\\E=1.275*10^{5} N/C

Part B:

Option B (Towards the South)

As electron is experiencing the force towards south,it means the direction of the electric field is towards the south because direction of field lines is from positive to negative, so proton is moving towards south it means negative charge is in south to which proton is attracted. So electric field is towards South.

7 0
3 years ago
A ramp leading to the entrance of a building is inclied upward at an angle of 7 degree. A suitcase is to be pulled up the ramp b
photoshop1234 [79]

Explanation:

The angle of the handle relative to the horizontal is 35°.  The angle of the ramp to the horizontal is 7°.  So the angle of the handle relative to the ramp is 28°.

cos 28° = 50 / F

F = 50 / cos 28°

F = 56.6 lbs

8 0
3 years ago
An elephant kicks a 2.5 kg rock that is initially at rest. The
erma4kov [3.2K]

0.20Answer:

Explanation:

6 0
3 years ago
The momentum of a 0.1 kg object traveling at 2000 m/s is 20,000 kg·m/s. True or False
Alina [70]

That's false.  

The definition of momentum is (mass) x (speed), so they must be multiplied.

"20,000 kg-m/s" has the correct units resulting from multiplication, but the number could only be the result of division.

3 0
3 years ago
The owner of a company that manufactures drinking cups decides it would be impressive to build an inground swimming pool that is
garri49 [273]

Answer:

The depth is 5.15 m.

Explanation:

Lets take the depth of the pool = h m

The atmospheric pressure ,P = 101235 N/m²

The area of the top = A m²

The area of the bottom =  a m²

Given that A= 1.5 a

The force on the top of the pool = P A

The total pressure on the bottom = P + ρ g h

ρ =Density of the  water = 1000 kg/m³

The total pressure at the bottom of the pool =  (P + ρ g h) a

The bottom and the top force is same

(P + ρ g h) a = P A

P a +ρ g h a =  P A

ρ g h a =  P A - P a

h=\dfrac{P ( A-a)}{\rho g a}

h=\dfrac{P ( 1.5 a-a)}{\rho g a}

h=\dfrac{P ( 1.5- 1)}{\rho g}

h=\dfrac{101235 ( 1.5- 1)}{1000\times 9.81}\ m

h=5.15 m

The depth is 5.15 m.

7 0
3 years ago
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