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xxMikexx [17]
2 years ago
12

What is the temperature used for the extension step?

Physics
1 answer:
lisabon 2012 [21]2 years ago
8 0
<h2>72°C</h2>

Explanation:

  • The polymerase chain response (PCR) could be a strategy to quickly open up groupings of DNA.
  • Amid a normal PCR, format DNA (containing the locale of intrigued) is blended with deoxynucleotides (dNTPs), a DNA polymerase and small segments of DNA called primers.
  • PCR involves a series of temperature cycles.
  • The temperature used for the extension step is 72°C.
  • The temperature that is used during the extension phase is dependent on the DNA polymerase that is used.
  • Amid the expansion step (ordinarily 68°C-72°C) the polymerase amplifies the groundwork to create an early DNA strand. This prepare is rehashed different times
  • Generally at this stage, the reaction mixture is heated to a temperature intermediate between the denaturation and annealing temperatures. The optimal temperature for Taq polymerase is 72°C.
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Don’t mind this i just need to answer under something because i just signed up !
4 0
3 years ago
What is perfect machine?Why is this machine not possible in the real life?​
andrezito [222]

Explanation:

The machine whose efficiency is 100% is known as perfect machine .This machine is not possible in real life because every machine is affected by the overcoming friction due to which is efficiency become less than hundred percent .

5 0
2 years ago
Two particles, one with charge -5.45 × 10^-6 C and one with charge 4.39 × 10^-6 C, are 0.0209 meters apart. What is the magnitud
Levart [38]

Explanation:

Given that,

Charge 1, q_1=-5.45\times 10^{-6}\ C

Charge 2, q_2=4.39\times 10^{-6}\ C

Distance between charges, r = 0.0209 m

1. The electric force is given by :

F=k\dfrac{q_1q_2}{r^2}

F=9\times 10^9\times \dfrac{-5.45\times 10^{-6}\times 4.39\times 10^{-6}}{(0.0209)^2}

F = -492.95 N

2. Distance between two identical charges, r=0.0209\ m

Electric force is given by :

F=\dfrac{kq_3^2}{r^2}

q_3=\sqrt{\dfrac{Fr^2}{k}}

q_3=\sqrt{\dfrac{492.95\times (0.0209)^2}{9\times 10^9}}

q_3=4.89\times 10^{-6}\ C

Hence, this is the required solution.

3 0
2 years ago
How does exhailing remove waste from the body? Explain the systems that make this happen‚ using complete sentences​
aliina [53]

Answer:

The lungs get rid of carbon dioxide and water vapor. The liver gets rid of bile, which, in addition to breaking down fats, is partially made up of the breakdown of red blood cells. The kidneys get rid of toxins from the blood. The large intestine gets rid of undigested food

3 0
2 years ago
Two sound waves have equal displacement amplitudes, but wave 1 has two-thirds the frequency of wave 2. What is the ratio of the
zlopas [31]

Answer:

\dfrac{I_1}{I_2}=\dfrac{4}{9}

Explanation:

c = Speed of wave

\rho = Density of medium

A = Area

\nu = Frequency

\nu_1=\dfrac{2}{3}\nu_2

Intensity of sound is given by

I=\dfrac{1}{2}\rho c(A\omega)^2\\\Rightarrow I=\dfrac{1}{2}\rho c(A2\pi \nu)^2

So,

I\propto \nu^2

We get

\dfrac{I_1}{I_2}=\dfrac{\nu_1^2}{\nu_2^2}\\\Rightarrow \dfrac{I_1}{I_2}=\dfrac{\dfrac{2}{3}^2\nu_2^2}{\nu_2^2}\\\Rightarrow \dfrac{I_1}{I_2}=\dfrac{4}{9}

The ratio is \dfrac{I_1}{I_2}=\dfrac{4}{9}

8 0
2 years ago
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