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3 years ago

What is the temperature used for the extension step?

Physics

1 answer:

lisabon 2012 [21]3 years ago

8 0

Explanation:

The polymerase chain response (PCR) could be a strategy to quickly open up groupings of DNA.
Amid a normal PCR, format DNA (containing the locale of intrigued) is blended with deoxynucleotides (dNTPs), a DNA polymerase and small segments of DNA called primers.
PCR involves a series of temperature cycles.
The temperature used for the extension step is 72°C.
The temperature that is used during the extension phase is dependent on the DNA polymerase that is used.
Amid the expansion step (ordinarily 68°C-72°C) the polymerase amplifies the groundwork to create an early DNA strand. This prepare is rehashed different times
Generally at this stage, the reaction mixture is heated to a temperature intermediate between the denaturation and annealing temperatures. The optimal temperature for Taq polymerase is 72°C.

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. Reem took a wire of length 10 cm. Her friend Nain took a wire of 5 cm of the same material and thickness both of them connecte

Umnica [9.8K]

Given :

Reem took a wire of length 10 cm. Her friend Nain took a wire of 5 cm of the same material and thickness both of them connected with wires as shown in the circuit given in figure. The current flowing in both the circuits is the same.

To Find :

Will the heat produced in both the cases be equal.

Solution :

Heat released is given by :

H = i²Rt

Here, R is resistance and is given by :

$R = \dfrac{\rho L}{A}$

So,

$H = i^2\times \dfrac{\rho L}{A} t\\\\H = \dfrac{i^2\rho Lt}{A}$

Now, in the question every thing is constant except for the length of the wire and from above equation heat is directly proportional to the length of the wire.

So, heat produced by Reem's wire is more than Nain one.

Hence, this is the required solution.

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3 years ago

In each of the parts of this question, a nucleus undergoes a nuclear decay. Determine the resulting nucleus in each case.

vaieri [72.5K]

Answer:

(A). The resulting nucleus is Fr.

(B). The resulting nucleus is Po.

(C). The resulting nucleus is Ne

(D). The resulting nucleus is Tc.

Explanation:

Given that,

A nucleus undergoes a nuclear decay.

(A). In alpha decay,

We know that,

When the nucleus emit alpha particle then atomic mass of particle reduce by 4 and atomic number reduce by 2.

We need to calculate the resulting nucleus

Using given data

$^{227}_{89}Ac\Rightarrow ^{227-4}_{89-2}X$

$^{227}_{89}Ac\Rightarrow ^{223}_{87}Fr$

The resulting nucleus is Fr.

(B). In beta-minus decay,

We know that,

When the nucleus emit beta- minus particle then atomic mass of particle is same and atomic number increase by 1.

We need to calculate the resulting nucleus

Using given data

$^{211}_{83}Bi\Rightarrow ^{211}_{83+1}X$

$^{211}_{83}Bi\Rightarrow ^{211}_{84}Po$

The resulting nucleus is Po.

(C). In beta-plus decay,

We know that,

When the nucleus emit beta- plus particle then atomic mass of particle is same and atomic number decrease by 1.

We need to calculate the resulting nucleus

Using given data

$^{22}_{11}Na\Rightarrow ^{22}_{11-1}X$

$^{22}_{11}Na\Rightarrow ^{22}_{10}Ne$

The resulting nucleus is Ne.

(D). In gamma decay,

We know that,

When the nucleus emit gamma particle then atomic mass and atomic number of particle is same.

We need to calculate the resulting nucleus

Using given data

$^{98}_{43}Tc\Rightarrow ^{98}_{43}Tc$

The resulting nucleus is Tc.

Hence, (A). The resulting nucleus is Fr.

(B). The resulting nucleus is Po.

(C). The resulting nucleus is Ne

(D). The resulting nucleus is Tc.

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3 years ago

3. A car has a mass of 2.50 x 10^3 kg. If the force acting on the car is 7.65 x 10^3 N to the

const2013 [10]

Answer:

3.06m/s² to the east

Explanation:

Given parameters:

Mass of car = 2.5 x 10³kg

Force acting on the car = 7.65 x 10³N

Unknown:

Acceleration of the car = ?

Solution:

From Newton's second law of motion:

Force = mass x acceleration

Acceleration = $\frac{Force }{mass}$ = $\frac{7.65 x 10^{3} }{2.5 x 10^{3} }$ = 3.06m/s² to the east

6 0

3 years ago