Answer: Total pressure of the gas will be 0.716atm.
Explanation: We are given a gas having initial conditions as
V = 27L
T = 32°C = 305K
P = 2.5atm
As the gas remains same, number of moles of a gas will also be same for initial and final conditions. To calculate the number of moles, we use ideal gas equation, which is,
.......(1)
where, R = gas constant = 
For calculating number of moles:

Putting the values of initial condition in this equation, we get

n = 2.696 mol
Now, the final conditions are,
V = 88.0L
T = 12°C = 285K
n = 2.696 mol (calculated above)
P = ? atm
Again using equation 1, we get


P = 0.716atm.
Phosphoenol pyruvate enzyme is not part of gluconeogenesis.
<h3>Phosphoenol pyruvate</h3>
The ester formed when pyruvate and phosphate are combined to form an enol results in phosphoenol pyruvate (2-phosphoenolpyruvate, or PEP). As an anion, it exists. In biochemistry, PEP is a crucial intermediary. Involved in glycolysis and gluconeogenesis, it boasts the highest-energy phosphate bond yet discovered in an organism (61.9 kJ/mol). It also plays a role in carbon fixation and the manufacture of a number of aromatic chemicals in plants. In bacteria, it provides energy for the phosphotransferase system. Enolase reacts with 2-phosphoglyceric acid to produce PEP as a result. Pyruvate kinase (PK) converts PEP to pyruvic acid, and this process produces adenosine triphosphate (ATP) via substrate-level phosphorylation. One of the main units of currency for chemical energy in cells is ATP.
Learn more about Phosphoenol pyruvate here:
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it doesn't have savanna climate which is mostly found in Africa content