3 years ago

An atomic spectra is like a _________ for an element, in that it is unique for that element and can be used in identification.

Chemistry

1 answer:

adell [148]3 years ago

3 0

Atomic number, because the atomic number is unque to each individua element.

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The molar absorptivity of a tyrosine residue at 280 nm is 2000 M-1cm-1, while for tryptophan it is 5500 M-1cm-1. A protein has b

adelina 88 [10]

Answer:

There are 4 tryptophans in the protein.

Explanation:

According to question, protein contains one tyrosine residue and say x number of tryptophans.

Concentration of protein solution = 1.0 micromolar = $1.0\times 10^{-6} Molar$

Molar absorptivity of a protein solution : $\epsilon$

$\epsilon = \epsilon _{tyro}+\epsilon _{tryp}$

$=1\times 2000 M^{-1}cm^{-1}+x\times 5500 M^{-1}cm^{-1}$

Length of the cuvette = l = 1.0 cm

Absorbance of protein solution at 280 nm = A = 0.024

$A=\epsilon \times l\times c$ ( Beer-Lambert's law)

$0.024=(1\times 2000 M^{-1}cm^{-1}+x\times 5500 M^{-1}cm^{-1})\times 1 cm\times 1.0\times 10^{-6} M$

Solving for x :

x = 4

There are 4 tryptophans in the protein.

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