Answer:
The correct/closest option is b
Explanation:
Restriction enzymes are enzymes (endonucleases) that cut short DNA strands at specific sites. Hence, each restriction enzyme has it's own specific site (between two bases) it cuts at. There are two types of end that can be produced by this cut; the blunt end and the sticky end.
A restriction enzyme recognizes (palindromic sequence) and cut in it's own specific end.
For example, if a restriction enzyme cuts between a guanine (G) and an adenine (A), and it cuts a palindromic double stranded DNA in the manner below, it produces a sticky end.
G║AATTC
CTTAA║G
And if a restriction enzyme cuts between guanine (G) and cytosine (C) in the manner below, it produces a blunt end.
GGG║CCC
CCC║GGG
Hence, from the question, restriction enzymes (although chosen by the scientist based on desired sequence to be cut) recognize the sticky or blunt ends itself.
Answer:
Explanation:
For ligation process the 1:3 vector to insert ratio is the good to utilize . By considering that we can take 1 ratio of vector and 3 ratio of insert ( consider different insert size ) and take 10 different vials of ligation ( each calculated using different insert size from low to high ) and plot a graph for transformation efficiency and using optimum transformation efficiency we can find out the insert size.
Answer:
6.5 × 10¹⁵/ cm³
Explanation:
Thinking process:
The relation 
With the expression Ef - Ei = 0.36 × 1.6 × 10⁻¹⁹
and ni = 1.5 × 10¹⁰
Temperature, T = 300 K
K = 1.38 × 10⁻²³
This generates N₀ = 1.654 × 10¹⁶ per cube
Now, there are 10¹⁶ per cubic centimeter
Hence, 
Answer:

Explanation:
Previous concepts
Angular momentum. If we consider a particle of mass m, with velocity v, moving under the influence of a force F. The angular momentum about point O is defined as the “moment” of the particle’s linear momentum, L, about O. And the correct formula is:

Applying Newton’s second law to the right hand side of the above equation, we have that r ×ma = r ×F =
MO, where MO is the moment of the force F about point O. The equation expressing the rate of change of angular momentum is this one:
MO = H˙ O
Principle of Angular Impulse and Momentum
The equation MO = H˙ O gives us the instantaneous relation between the moment and the time rate of change of angular momentum. Imagine now that the force considered acts on a particle between time t1 and time t2. The equation MO = H˙ O can then be integrated in time to obtain this:

Solution to the problem
For this case we can use the principle of angular impulse and momentum that states "The mass moment of inertia of a gear about its mass center is
".
If we analyze the staritning point we see that the initial velocity can be founded like this:

And if we look the figure attached we can use the point A as a reference to calculate the angular impulse and momentum equation, like this:

](https://tex.z-dn.net/?f=0%2B%5Csum%20%5Cint_%7B0%7D%5E%7B4%7D%2020t%20%280.15m%29%20dt%20%3D0.46875%20%5Comega%20%2B%2030kg%5B%5Comega%280.15m%29%5D%280.15m%29)
And if we integrate the left part and we simplify the right part we have

And if we solve for
we got:
