Answer: <u>Endonuclease enzymes used in molecular biology that cut DNA at specified points.</u>
Explanation:
Enzymes are specific protein types which bind to a substrate within a reaction, to increase the rate of reaction within the solution- they speed up the rate of reaction.
Restriction enzymes are bacteria-derived enzymes; these make cuts on deoxyribonucleic acid molecules or DNA. These are also called restriction endonucleases. They are utilized in molecular biology for DNA cloning and sequencing and cut DNA into smaller pieces called fragments.
Restriction enzymes make directed cuts on DNA molecules. They precisely target sites on DNA to produce mostly identical or homogenous, discrete fragments of equal sizes, producing blunt or sticky ends. In order to do this, they recognize sequences of nucleotides that correspond with a complementary sequence on the endonuclease called restriction sites.
There are several kinds that may require cofactors (chemical or metallic compounds that aid in enzyme activity) :
- Type I: cleave far away from the recognition site; require ATP and SAMe S-Adenosyl-L-Methionine
- Type II: cleave near to the site; require Magnesium
- Type III: cleave near to the site; require ATP which is not hydrolysed but SAMe S-Adenosyl-L-Methionine is optional
- Type IV: cleavage targeted to DNA that have undergone post transcriptional modification through certain types of methylation (addition of a methyl group)
Answer: a. two substances present; two phases present : Heterogeneous mixture
b. two substances present; one phase present
: Homogeneous mixture
c. one substance present; one phase present
: pure substance.
d. one substance present; two phases present: Heterogeneous mixture
Explanation:
A pure substance is a substance which contains definite composition of only one type of component. Hence, it cannot be separated by physical means.
Mixture is a substance which contains two or more than two types of components and they can be separated by physical means as well.
Homogeneous mixtures: It is a mixture that has uniform composition throughout the solution and the particle size or shapes are not different. There is no physical boundary between the dispersed phase and dispersion medium.
Heterogeneous mixtures: It is a mixture that has non-uniform composition throughout the solution and the particle size or shapes are also different. There is a physical boundary between the dispersed phase and dispersion medium.
Answer: The correct option is C ( is very hard and burns cleanly).
Explanation:
COAL is a form of rock that is made up of mostly carbon amongst other elements which includes sulphur, nitrogen, hydrogen and oxygen. There are different types of coal which include:
--> anthracite ( 90% carbon)
--> bituminous coal ( 70-90% carbon)
--> lignite ( 60- 70% carbon) and
--> peat (60 % carbon).
Anthracite is the type of coal that contains the highest carbon content ( 90% carbon). This makes it very hard and is often a times referred to as HARD COAL. Anthracite is a higher quality coal for domestic and open fire heating. This is because it contains less impurities than other type of coal and thereby making it to BURN CLEANLY avoiding atmospheric pollution.
Answer:
It reads as follows: 0.5 moles of solute per liter of solution.
Explanation:
Molarity is the most frequent way of expressing the concentration of solutions in chemistry, and it indicates the number of moles of solute dissolved per liter of solution; is represented by the letter M.