The loss or gain of electrons
Explanation:
The loss or gain of electrons determines if an atom will become a cation or anion.
A cation is a positively charge ion
An anion is a negatively charged ion.
In an atom, we have sub-atomic particles:
Protons are the positively charged particles
Electrons are negatively charged
Neutrons carry no charges
Only electrons can be lost or gained in chemical processes that forms cations and anions.
When a neutral atom gains electron, it has more electrons than protons. This makes it negatively charged and we call it an anion.
When a neutral atom loses an electron, the number of protons is more. We call it a cation.
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Alkali are soluble bases, however not all bases are soluble in water, therefore not all bases are Alkali.
0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.
<h3>What is dilution?</h3>
Dilution is a process of making a solution of lower concentration from a solution of higher concentration by the addition of solvent to a given volume of the solution of higher concentration.
Dilution of solutions is done using the dilution formula in order to determine the given volume of diluent or stock solution required. The dilution formula is given below:
where:
- C1 = Initial concentration of enzyme
- C2 = Final concentration of enzyme
- V1 = Initial volume
- V2 = Final volume
For the enzyme dilution;
C1 = 1 mg/mL
C2 = 1/50 mg/ml = 0.02 mg/ml
V= ?
V2 = 5 ml
V1 = C2V2/C1
V1 = 0.02 * 5/1 = 0.1 mL
Therefore, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.
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