Answer:
A) That resistance in bacteria is produced due to inactivation of ampicillin by the beta lactamase enzyme. This enzyme is expressed by the bla gene found in the plasmid. This enzyme is secreted into the culture medium, thereby inactivating ampicillin. Thanks to this inactivation, the bacteria colonies will be able to develop. After a day of incubation, only those bacteria that took the plasmid that gives them resistance to ampicillin will grow after transformation. After prolonged incubation, two types of colonies can be observed in the culture medium. One large colony with ampicillin resistance, and another small colony, both of which are sensitive to ampicillin.
B) Large colonies are characterized by being resistant to ampicillin. When Ramón isolates the plasmid, he will have the gene that provides resistance to antibiotics. Said plasmid can be used again on those bacteria that are sensitive to ampicillin.
On the other hand, satellite colonies are sensitive to ampicillin. These types of colonies do not have the plasmid that contains the gene that gives ampicillin resistance. It is not possible to isolate any plasmids from these satellite colonies. These satellite bacteria will not be able to grow if they are transferred to a plate containing fresh ampicillin, while large colonies, which possess the plasmid that gives them resistance to ampicillin, will be able to grow on that plate.
Explanation:
When the Pka for formic acid = 3.77
and Pka = -㏒ Ka
3.77 = -㏒ Ka
∴Ka = 1.7x10^-4
when Ka = [H+][HCOO-}/[HCOOH]
when we have Ka = 1.7x10^-4 &[HCOOH] = 0.21 m
so by substitution: by using ICE table value
1.7x10^-4 = X*X / (0.21-X)
(1.7x10^-4)*(0.21-X) = X^2 by solving this equation for X
∴X = 0.0059
∴[H+] = 0.0059
∴PH= -㏒ [H+]
= -㏒ 0.0059
= 2.23
answer:
in plants
Transport manufactured food from the leaves to others parts of the plant
Facilitates gaseous exchange through the stomata in the leaves to other parts of the plant
in animal
Exchange of respiratory gases across respiratory services
Excretion of nitrogenous waste in some unicellular organisms
Explanation:
Hope it benefit
A) At 0 C water forms ice but as mentioned above F) water's greatest density occurs at 4 C and it decreases below 4 C so ice is lighter than 4C water, thus, at 0C ice comes to surface and acts as insulator thereby preventing lower water from freezing.
Answer:
Alcohol will form at more substituted carbon due to carbocation rearrangement so 2-hexanol
Explanation:
Well if it’s acid catalyzed
