Answer:
Explanation:
For ligation process the 1:3 vector to insert ratio is the good to utilize . By considering that we can take 1 ratio of vector and 3 ratio of insert ( consider different insert size ) and take 10 different vials of ligation ( each calculated using different insert size from low to high ) and plot a graph for transformation efficiency and using optimum transformation efficiency we can find out the insert size.
Answer: Rupture strength
Explanation: Rupture strength is the strength of a material that is bearable till the point before the breakage by the tensile strength applied on it. This term is mentioned when there is a sort of deformation in the material due to tension.So, rupture will occur before whenever there are chances of failing and the material is still able to bear stresses before failing.
Answer:
diameter is 14 mm
Explanation:
given data
power = 15 kW
rotation N = 1750 rpm
factor of safety = 3
to find out
minimum diameter
solution
we will apply here power formula to find T that is
power = 2π×N×T / 60 .................1
put here value
15 ×
= 2π×1750×T / 60
so
T = 81.84 Nm
and
torsion = T / Z ..........2
here Z is section modulus i.e = πd³/ 16
so from equation 2
torsion = 81.84 / πd³/ 16
so torsion = 416.75 / / d³ .................3
so from shear stress theory
torsion = σy / factor of safety
so here σy = 530 for 1020 steel
so
torsion = σy / factor of safety
416.75 / d³ = 530 × 
/ 3
so d = 0.0133 m
so diameter is 14 mm
Answer:
By definition the ultimate tensile strength is the maximum stress in the stress-strain deformation. The stress at 0.2% strain, the stress at the onset of plastic deformation, the stress at the end of the elastic deformation and the stress at the fracture correspond, by definition, to other points of the stress-strain curve.
Explanation:
