I think you forgot to attach the diagram along with the question. I am answering the question based on my research and knowledge. "Count the number of protons" is the one among the following choices given in the question by which the <span>atomic number of nitrogen be determined. The correct option among all the options that are given in the question is the second option or option "B".</span>
The Ninhydrin test is not effective to detect high molecular weight proteins as the steric hindrance limits the ninhydrin from reaching the α-amino groups.