As described in "A Note About Bacterial Reproduction -- and the "Culture Bias,"" the organism Epulopisciumdoes not divide by bin
ary fission. Rather, each cell increases in sizeand divides to produce multiple daughter cells that are held within the original cell well.After lysis, those daughters are released to repeat the process. Assuming Epulopisciumcould be grown in pure culture in the laboratory in broth and on solid media (it currently cannot), which method would be best for measuring the increase in biomass during growth?Select one:a. Turbidity readings from a spectrophotometer; the increase in biomass will directly vary with the turbidity of the culture. b. The viable plate count; each colony derives from a single cell and the number of coloniesequals the number of cells. c. Direct microscopic count; the experimenter can directly count the number of cells and extrapolate to the biomass.d. Chemostat growth; it will prevent the culture from entering the death phase
The best method that will yield significantly more accurate result is to use spectrophotometer to read the turbidity of the sample and increase in turbidity is associated with increase biomass.
