Question

. We measure the absorbance of a protein solution with the concentration 0.04 mM, which absorbs at 595nm. The cuvette has a width of 1cm and ε595=14000M-1cm-1. What is the absorbance?

Answer 1

Answer:

0.56 is the absorbance of a protein solution with the concentration 0.04 mM, which absorbs at 595nm.

Explanation:

Concentration of protein solution = C 0.04 mM  = $0.04\times 0.001 M$

1 mM = 0.001 M

Length of cuvette = l = 1 cm

Molar absorptivity coefficient of the solution = $\epsilon=14000 M^{-1}cm^{-1}$

Absorbance of a protein solution = A

Using Beer-Lambert's law :

Formula used :

$A=\epsilon \times C\times l$

$A=14000 M^{-1}cm^{-1}\times 0.04 \times 0.001 M\times 1 cm$

A = 0.56

0.56 is the absorbance of a protein solution with the concentration 0.04 mM, which absorbs at 595nm.