The particles that carry charge through wires in a circuit are mobile electrons. The electric field direction within a circuit is by definition the direction that positive test charges are pushed. Thus, these negatively charged electrons move in the direction opposite the electric field.
Isotopes refer to different atoms of the same element (i.e. same number of protons) that differ in the number of neutrons they have (giving them different atomic weights). Atomic weight is the sum of protons and neutrons (each contributes 1 atomic mass unit).
Carbon has 6 protons by definition. If you have a carbon-13 atom (the 13 referring to its mass), the atom has 13 - 6 = 7 neutrons. Since it's neutral, protons = electrons, so there are also 6 electrons.
Sulfur has 16 protons by definition. If you have a sulfur-32 atom, the atom has 32 - 16 = 16 neutrons. Since it's neutral, protons = electrons, so there are also 16 electrons.
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