<span>Based on the experience of the responder, to correctly calculate measurements in real-world. Firstly is to avoid errors as much as possible. Errors are what makes your measurement invalid and unreliable. There are two types of error which is called the systematic error and the random error. Each error has different sources. Words that were mentioned –invalid and unreliable are very important key aspects to determine that your measure is truly accurate and consistent. Some would recommend using the mean method, doing three trials in measuring and getting their mean, in response to this problem.</span>
Explanation:
According to the given data, we will calculate the following.
Half life of lipase
= 8 min x 60 s/min
= 480 s
Rate constant for first order reaction is as follows.
=
Initial fat concentration
= 45
= 45 mmol/L
Rate of hydrolysis
= 0.07 mmol/L/s
Conversion X = 0.80
Final concentration (S) =
= 45 (1 - 0.80)
= 9
or, = 9 mmol/L
It is given that
= 5mmol/L
Therefore, time taken will be calculated as follows.
t = ![-\frac{1}{K_{d}}ln[1 - \frac{K_{d}}{V}{K_{M} ln (\frac{S_{o}}{S}) + (S_{o} - S)]](https://tex.z-dn.net/?f=-%5Cfrac%7B1%7D%7BK_%7Bd%7D%7Dln%5B1%20-%20%5Cfrac%7BK_%7Bd%7D%7D%7BV%7D%7BK_%7BM%7D%20ln%20%28%5Cfrac%7BS_%7Bo%7D%7D%7BS%7D%29%20%2B%20%28S_%7Bo%7D%20-%20S%29%5D)
Now, putting the given values into the above formula as follows.
t =
= ![-\frac{1}{1.44 \times 10^{-3}s^{-1}}ln[1 - \frac{1.44 \times 10^{-3}s^{-1}}{0.07 mmol/L/s }{K_{M} ln (\frac{45 mmol/L }{9 mmol/L }) + (45 mmol/L - 9 mmol/L )]](https://tex.z-dn.net/?f=-%5Cfrac%7B1%7D%7B1.44%20%5Ctimes%2010%5E%7B-3%7Ds%5E%7B-1%7D%7Dln%5B1%20-%20%5Cfrac%7B1.44%20%5Ctimes%2010%5E%7B-3%7Ds%5E%7B-1%7D%7D%7B0.07%20mmol%2FL%2Fs%0A%7D%7BK_%7BM%7D%20ln%20%28%5Cfrac%7B45%20mmol%2FL%0A%7D%7B9%20mmol%2FL%0A%7D%29%20%2B%20%2845%20mmol%2FL%20-%209%20mmol%2FL%0A%29%5D)
= 
= 27.38 min
Therefore, we can conclude that time taken by the enzyme to hydrolyse 80% of the fat present is 27.38 min.
It depends on the pH if the base. but normally light colors are for bases example blue green etc