Why is wavelength the main limiting factor on limit of resolution in light microscopy?

1 answer:

3 0

The resolution of a microscope is the smallest resolvable distance between two objects. It is defined as the shortest distance between two points on a specimen and the observer can still distinguish them.
The wavelength is a determining and limiting factor in the degree of resolution afforded by the microscope. The relationship between the wavelength and the resolution is:
Shorter wavelengths yield higher resolution and visa versa.

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A copper (Young's modulus 1.1 x 1011 N/m2) cylinder and a brass (Young's modulus 9.0 x 1010 N/m2) cylinder are stacked end to end, as in the drawing. Each cylinder has a radius of 0.24 cm.

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